Newcastle disease vaccine Yupingfeng polysaccharide immunopotentiator

An immune enhancer, jade screen technology, applied in the field of livestock and poultry immunity, can solve the problems of lack of immune enhancer, short duration of efficacy, long medication time, etc., to enhance animal immune function, promote value, and improve disease resistance. Effect

Active Publication Date: 2015-12-16
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the shortcomings of chemical drugs such as long medication time, short duration of curative effect, drug residues, and environmental pollution, and the immune response of animals has an extremely complicated process and the participation of various factors, when immunosuppression occurs, it is often not a single one. Chemical immune enhancers can work, so there is a special lack of highly effective and low-toxic immune enhancers

Method used

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  • Newcastle disease vaccine Yupingfeng polysaccharide immunopotentiator
  • Newcastle disease vaccine Yupingfeng polysaccharide immunopotentiator
  • Newcastle disease vaccine Yupingfeng polysaccharide immunopotentiator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 polysaccharide

[0030] Based on 1000g of Yupingfeng Powder, astragalus, Atractylodes macrocephala, and Fangfeng are configured according to the mass ratio of 3:1:1 to make 1000g of Yupingfeng Powder, add 8 times the quality of water and soak for 30 minutes; then boil for 1 hour, add 6 times the quality of the medicinal residue after filtering Boil water for 1 hour, add 6 times the quality of water to the filter residue, boil for 1 hour and filter, combine the filtrate three times, heat and concentrate the filtrate to 1000mL, after cooling, centrifuge at 3000rpm for 10min, remove impurities, distill water to make 1000mL; use one-step alcohol precipitation method Extraction, slowly add 95wt% ethanol to the above-mentioned concentrated solution to make the final concentration of ethanol in the mixed solution reach 80wt%, stir while adding, let stand for 24 hours after the addition is completed, centrifuge, take the precipitate and place it i...

Embodiment 2

[0031] The purification of embodiment 2 polysaccharides

[0032] Take YPF-PS respectively tc Dissolve 500mg in 10mL of distilled water to obtain 50mg.mL -1 Sample, followed by chromatography column (1.6cm×90cm), the medium is SephadexA-25, the flow rate is 0.42mL min -1 Load the sample, and then collect it in fractions, using 0.05mol·L -1 NaCl and 1mol L -1 Gradient elution with 400mL of NaCl each, and the eluate was dialyzed through a dialysis bag to remove sodium chloride and impurities to obtain purified polysaccharide (YPF-PS tp ).

Embodiment 3

[0033] The comparison of embodiment 3 in vitro immune effects

[0034] Method: Yupingfeng polysaccharide was used as test material, and RAMPS tc , RAMPS tp , Atractylodes macrocephala graded polysaccharides (RAMPS 60c , RAMPS 70c and RAMPS 80c) as comparison. Firstly, the safe concentrations of the three prescriptions on peripheral blood lymphocytes were determined, and then the three prescriptions were diluted with cell culture medium (RPMI1640) to 62.5, 31.25, 15.625, 7.813, and 3.907 μg·mL -1 A total of 5 working concentrations were added to the cultured chicken peripheral blood lymphocytes respectively, and the absorbance value at 570nm was detected on an enzyme-linked immunosorbent analyzer (A 570value), as an indicator of lymphocyte proliferation. And calculate the highest cell proliferation rate of each polysaccharide according to the formula: the highest cell proliferation rate=(the highest A of the polysaccharide group 570 Value - Cell Control A 570 value) / cel...

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Abstract

The invention belongs to the field of livestock and poultry immunity, provides a livestock and poultry immunoadjuvant, and particularly discloses a Newcastle disease vaccine Yupingfeng polysaccharide immunopotentiator. The immunopotentiator is mainly prepared with astragalus, white atractylodes rhizome and Chinese parsnip root according to a mass ratio of 3:1:1 through a water decoction-alcohol precipitation method, and an important ingredient of the immunopotentiator is Yupingfeng polysaccharide. The immunopotentiator is adopted to be matched with vaccines for use, immunity of animals can be enhanced, and disease resistance of the animals and protection rate of the vaccines are improved, so that the immunopotentiator has high popularization value in preventing and controlling infectious diseases of the animals.

Description

technical field [0001] The invention belongs to the field of livestock and poultry immunity, provides a livestock and poultry immune adjuvant, and specifically discloses a Newcastle disease vaccine traditional Chinese medicine Yupingfeng polysaccharide immune enhancer. Background technique [0002] With the development of China's economy and the rapid growth of animal husbandry, animal diseases not only cause huge economic losses to animal husbandry production, but also some zoonotic diseases directly threaten the survival and development of human beings. It is now recognized that disorders of the body's immune system are more susceptible to infection. However, due to the shortcomings of chemical drugs such as long medication time, short duration of curative effect, drug residues, and environmental pollution, and the immune response of animals has an extremely complicated process and the participation of various factors, when immunosuppression occurs, it is often not a singl...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61K39/39A61P37/04A61P31/14A61K36/481
Inventor赵晓娜孙文静刘建柱马藤菲郝盼王胜华
OwnerSHANDONG AGRICULTURAL UNIVERSITY