Carboxylated three-dimensional ordered mesoporous carbon-lysozyme composite material and its preparation method and application
A three-dimensional ordered, composite material technology, applied in chemical instruments and methods, fixed on or in inorganic carriers, water/sludge/sewage treatment, etc., can solve the problem that lysozyme is not reusable and bacteria are not easily captured , the adsorption capacity is not much and other problems, to achieve the effects of good hydrothermal stability and adsorption, improved activity, and excellent reusability
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Embodiment 1
[0055] A carboxylated three-dimensional ordered mesoporous carbon-lysozyme composite material uses carboxylated three-dimensional ordered mesoporous carbon as a carrier, and lysozyme is covalently cross-linked on the carboxylated three-dimensional ordered mesoporous carbon through a cross-linking agent. Cross-linking agents include N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide.
[0056] The preparation method of the carboxylated three-dimensional ordered mesoporous carbon-lysozyme composite material of this embodiment comprises the following steps:
[0057] (1) Preparation of carboxylated three-dimensional ordered mesoporous carbon
[0058] 1.1. Synthesis of ordered mesoporous silicon templates: First, dissolve 0.3 g lysine in 286 g deionized water, then add 22 g tetraethyl orthosilicate, heat and stir in an oil bath for 48 h, the stirring speed is 500 rpm, and the temperature Controlled at 60°C, a mixed solution was obtained. The resu...
Embodiment 2
[0068] The effects of different concentrations of lysozyme on the enzymatic activity of carboxylated three-dimensional ordered mesoporous carbon-lysozyme composites were investigated:
[0069] (1) Preparation Preparation of carboxylated three-dimensional ordered mesoporous carbon: same as in Example 1.
[0070] (2) Activation of carboxylated three-dimensional ordered mesoporous carbon: same as Example 1.
[0071] (3) Set 7 groups containing 10 mg of activated carboxylated three-dimensional ordered mesoporous carbon, and put it into 10 mL of 0.5 mg / mL, 1.0 mg / mL, 1.5 mg / mL, 2.0 mg / mL, 2.5 mg / mL, 3.0 mg / mL, 4.0 mg / mL lysozyme solution (pH=10), shake at room temperature 200 rpm for 9 h, filter and wash with phosphate buffer solution, then dry in a freeze dryer to obtain carboxylated Three-dimensional ordered mesoporous carbon-lysozyme composites.
[0072] The enzymatic activity of seven groups of carboxylated three-dimensional ordered mesoporous carbon-lysozyme composites was ...
Embodiment 3
[0074] The effects of different fixation times on the enzymatic activity of carboxylated three-dimensional ordered mesoporous carbon-lysozyme composites were investigated:
[0075] (1) Preparation Preparation of carboxylated three-dimensional ordered mesoporous carbon: same as in Example 1.
[0076] (2) Activation of carboxylated three-dimensional ordered mesoporous carbon: same as Example 1.
[0077] (3) Set up 6 groups containing 10 mg of activated carboxylated three-dimensional ordered mesoporous carbon, put it into 10 mL of lysozyme solution with a concentration of 3.0 mg / mL, adjust the pH to 10 to obtain mixed solutions 1~6, and mix solution 1 ~6 were shaken at room temperature at 200 rpm for 3 h, 6 h, 9 h, 12 h, 18 h, and 24 h to complete the fixation. Then filter and wash with phosphate buffer solution and dry in a freeze dryer to obtain a carboxylated three-dimensional ordered mesoporous carbon-lysozyme composite material.
[0078] The enzyme activities of six groups...
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