A method for preparing fungal hyperbranched polysaccharide-xanthan gum hydrogel scaffold

A technology of xanthan gum and polysaccharides, applied in medical science, prosthesis, etc., can solve the problems of fragility, poor water resistance and mechanical properties, and easy solubility in water, etc., and achieve low cost, improved drug efficacy, and embedding volume big effect

Inactive Publication Date: 2017-10-24
WUHAN TEXTILE UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problems of xanthan gum physical hydrogel are: easy to dissolve in water, poor water resistance and mechanical properties, easy to damage
The disadvantage of this method is that the cross-linking agent used is Ca 2+ , relying on the electrostatic force to form a cross-linked network, resulting in a physical hydrogel

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for preparing fungal hyperbranched polysaccharide-xanthan gum hydrogel scaffold
  • A method for preparing fungal hyperbranched polysaccharide-xanthan gum hydrogel scaffold
  • A method for preparing fungal hyperbranched polysaccharide-xanthan gum hydrogel scaffold

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] A method for preparing fungal hyperbranched polysaccharide-xanthan gum hydrogel scaffold, said preparation method comprising the following steps:

[0021]a. The dried sclerotia of tiger milk mushroom was crushed, and the fat was removed by Soxhlet extraction with ethyl acetate and acetone for 6 hours in sequence. Both ethyl acetate and acetone were chemically pure reagents. Then soak the sclerotium of tiger milk mushroom after fat removal in physiological saline at a temperature of 80°C for 2 hours, and centrifuge; the residue is soaked at a high pressure of 120°C for 30 minutes, wherein every 100g of tiger milk mushroom sclerotia is soaked in 1L of physiological saline, Centrifuge at 8000 rpm for 20 minutes to obtain the extract, cool the extract and then centrifuge and collect the residue; the residue is centrifugally washed with deionized water and freeze-dried to obtain the highly branched polysaccharide of tiger milk mushroom, which can also be dried by other method...

Embodiment 1

[0027] Crush 500g of dried tiger milk mushroom sclerotia, perform Soxhlet extraction with ethyl acetate and acetone for 6 hours to remove fat, then soak the fat-free tiger milk mushroom sclerotia in 5L of normal saline at 80°C for 2 hours, Centrifuge, soak the residue in 5L of normal saline at a high pressure of 120°C for 30 minutes, centrifuge to obtain an extract, cool the extract and then centrifuge to collect the residue, centrifuge the residue with deionized water and freeze-dry to obtain the highly branched polysaccharide of tiger milk mushroom. Dissolve 0.5g of tiger milk mushroom hyperbranched polysaccharide in 25mL of NaOH aqueous solution with pH=12, and prepare a solution of tiger milk mushroom hyperbranched polysaccharide with a concentration of 2% w / v after magnetic stirring for 2h. Dissolve 0.125g of xanthan gum Add it into the above-mentioned tiger milk mushroom hyperbranched polysaccharide solution with a concentration of 2% w / v, continue magnetic stirring for 1...

Embodiment 2

[0029] Crush 500g of dried tiger milk mushroom sclerotia, perform Soxhlet extraction with ethyl acetate and acetone for 6 hours to remove fat, then soak the fat-free tiger milk mushroom sclerotia in 5L of normal saline at 80°C for 2 hours, Centrifuge, soak the residue in 5L of normal saline at a high pressure of 120°C for 30 minutes, centrifuge to obtain an extract, cool the extract and then centrifuge to collect the residue, wash the residue with deionized water and freeze-dry to obtain highly branched tiger milk mushroom polysaccharide. Dissolve 0.5g of tiger milk mushroom hyperbranched polysaccharides in 25mL of NaOH aqueous solution with pH=13, and prepare a solution of tiger milk mushroom hyperbranched polysaccharides with a concentration of 2% w / v after magnetic stirring for 2 hours. Dissolve 0.25g of xanthan gum Add it into the above-mentioned tiger milk mushroom hyperbranched polysaccharide solution with a concentration of 2% w / v, and continue magnetic stirring for 12 h...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for preparing fungal hyperbranched polysaccharide-xanthan gum hydrogel scaffold, which belongs to the technical field of natural polymer materials. The preparation method adopts dissolving the highly branched polysaccharide and xanthan gum of tiger milk mushroom in different mass ratios in NaOH aqueous solution, and the obtained tiger milk mushroom hyperbranched polysaccharide and xanthan gum solution are mixed with sodium trimetaphosphate aqueous solution at a temperature of 37 ° C. After 10 minutes to 48 hours of joint reaction, the fungal hyperbranched polysaccharide-xanthan gum hydrogel scaffold was obtained. The method of the invention is easy to operate, the sources of raw materials used are abundant, the cross-linking agent used is water-soluble and non-toxic, and the prepared scaffold material has drug-controllable release properties, good mechanical properties and biocompatibility. The hyperbranched polysaccharide-xanthan gum hydrogel scaffold can be used to prepare artificial tissue scaffolds, food or nutrients, and drug controlled release carriers.

Description

technical field [0001] The invention relates to a method for preparing fungal hyperbranched polysaccharide-xanthan gum hydrogel scaffold. Belonging to the technical field of natural polymer materials, the fungal highly branched polysaccharide-xanthan gum hydrogel three-dimensional penetrating porous scaffold can be widely used in industries such as tissue engineering materials, biomedical materials, and drug controllable release. Background technique [0002] Polymer hydrogel has a certain degree of elasticity and a three-dimensional through-porous structure, which is suitable for simulating the extracellular matrix, providing a three-dimensional microenvironment suitable for cell growth and biophysical signals between cells and the extracellular matrix, and maintaining the normal phenotype and physiology of cells. Features. Polysaccharides are biological macromolecules that widely exist in nature. They participate in various life activities of cells and produce a variety o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/52A61L27/56A61L27/54A61L27/20A61L27/12A61K47/36
Inventor 陶咏真张如权徐卫林王哲柏自奎周应山
Owner WUHAN TEXTILE UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap