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Preparation method for producing heparin sodium by animal small intestines

A technology of heparin sodium and small intestine, which is applied in the field of preparation of isolated biochemical drugs, can solve problems such as complex procedures, failure to reach industrialized production, and environmental pollution by waste water, and achieve the goal of reducing production costs, reducing activity loss, and increasing yield Effect

Inactive Publication Date: 2015-12-30
NANTONG RENSHOU FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield of heparin sodium is low, the activity is not stable enough, the actual operation process is relatively strict and complicated, and it cannot meet the requirements of industrial production, so that the utilization of sheep small intestinal mucosa is unreasonable for a long time, and a large amount of heparin will be produced during the production process. Wastewater pollutes the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method for producing heparin sodium from the small intestine of animals. The preparation of heparin sodium is completed through the steps of preparing intestinal mucosa, enzymatic hydrolysis, adsorption, elution, precipitation and desalination, and drying; the specific steps are as follows:

[0019] (1) Preparation of intestinal mucosa: Pour clean water into the small intestine of the animal, and use a casing machine to squeeze out the small intestinal mucosa to the mucosa pool;

[0020] (2) Enzymolysis: use the mucous membrane pump to pump the mucous membrane from the mucous membrane pool into the enzymolysis tank, then pour water into the enzymolysis tank, the mass ratio of water to mucous membrane is 8:1, stir well and add an appropriate amount of salt , adjust the pH value to 7, the mass ratio of salt to mucous membrane is 1:500, heat to make the temperature in the enzymolysis tank reach 50°C, then add 4% salt, keep warm for 2 hours, continue heating to...

Embodiment 2

[0026] A preparation method for producing heparin sodium from the small intestine of animals. The preparation of heparin sodium is completed through the steps of preparing intestinal mucosa, enzymatic hydrolysis, adsorption, elution, precipitation and desalination, and drying; the specific steps are as follows:

[0027] (7) Preparation of intestinal mucosa: Pour clean water into the small intestine of the animal, and use a casing machine to squeeze out the small intestinal mucosa to the mucosa pool;

[0028] (8) Enzymolysis: use the mucous membrane pump to pump the mucous membrane from the mucous membrane pool into the enzymolysis tank, then pour water into the enzymolysis tank, the mass ratio of water to mucous membrane is 10:1, stir well and add an appropriate amount of salt , adjust the pH value to 8, the mass ratio of salt to mucous membrane is 1:500, heat to make the temperature in the enzymolysis tank reach 55°C, then add 6% salt, keep warm for 3 hours, continue heating t...

Embodiment 3

[0034] A preparation method for producing heparin sodium from the small intestine of animals. The preparation of heparin sodium is completed through the steps of preparing intestinal mucosa, enzymatic hydrolysis, adsorption, elution, precipitation and desalination, and drying; the specific steps are as follows:

[0035] (13) Preparation of intestinal mucosa: Pour clean water into the small intestine of the animal, and use a casing machine to squeeze out the small intestinal mucosa to the mucosa pool;

[0036] (14) Enzymolysis: Use a mucous membrane pump to pump the mucous membrane from the mucous membrane pool into the enzymolysis tank, then pour water into the enzymolysis tank, the mass ratio of water to mucous membrane is 9:1, stir well and add an appropriate amount of salt , adjust the pH value to 7.5, the mass ratio of salt to mucous membrane is 1:500, heat to make the temperature in the enzymolysis tank reach 53°C, then add 5% salt, keep warm for 2.5 hours, continue heatin...

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Abstract

The invention discloses a preparation method for producing heparin sodium by animal small intestines. The preparation method is characterized in that the preparation of the heparin sodium is completed through steps of intestinal mucosa preparation, enzymolysis, adsorption, elution, sedimentation and desalination and drying. The preparation method disclosed by the invention has the advantages that the yield of the heparin sodium is effectively increased by reasonably regulating the preparation method, and the loss of activity of the heparin sodium is reduced; the prepared heparin sodium is more stable in quality; only 1400 to 1500 animal small intestines are needed by producing 100 million units of heparin sodium by adopting the preparation method disclosed by the invention, so that the production cost is greatly reduced.

Description

technical field [0001] The invention relates to a preparation method for isolating biochemical drugs from animal mucous membranes, in particular to a preparation method for producing heparin sodium from the small intestine of animals. Background technique [0002] Heparin sodium is a mucopolysaccharide sulfate anticoagulant drug. It is the sodium salt of aminodextran sulfate extracted from the intestinal mucosa of pigs or cattle. It belongs to mucopolysaccharides and naturally exists in mast cells. It is widely used in the treatment of arterial and venous thrombosis and pulmonary embolism, ischemic stroke, unstable angina, acute myocardial infarction, etc. At present, heparin sodium is mainly extracted from the small intestinal mucosa of pigs and sheep, bovine lungs, and salted casing saline. Heparin sodium is a natural anticoagulant substance and is one of the main export drugs in my country. With the deepening of research, it has been found that heparin sodium not only ha...

Claims

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Application Information

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IPC IPC(8): C08B37/10
Inventor 冯爱国
Owner NANTONG RENSHOU FOOD CO LTD
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