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Node application method of medium-concentration IAA and glycerogel

A glycerin gel and concentration technology, applied in the field of plant biology, can solve the problems of branch loss, high cost, and many technical steps, and achieve the effect of reducing water evaporation and easy operation

Inactive Publication Date: 2016-01-06
钱夕刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] A method for using medium-concentration IAA and glycerin gel at nodes, characterized in that the method includes hormone mother solution preparation, working solution preparation, hormone gel preparation, cuttings, and dripping at axillary buds. The specific operation steps are as follows:

[0014] ① Hormone mother solution preparation: Accurately weigh 0.1g of IAA and dissolve it with a little 95% ethanol, then dilute to 100mL with distilled water, which is 1mg / mL IAA mother solution;

[0015] ② Preparation of working solution: Dilute the above mother solution according to the volume ratio of 1:10 into a medium-concentration hormone working solution with a concentration of 100mg / L, and add 10ml of glycerin to 90ml of hormone working solution at the same time;

[0016] ③ Hormone gel preparation: Accurately weigh 1 g of sodium carboxymethylcellulose and 8 g of sodium alginate, and dissolve them in 100 mL of hormone and glycerin working solution with a concentration of 100...

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Abstract

The invention discloses a node application method of medium-concentration IAA and glycerogel. The method comprises the following steps: 1mg / mL of IAA mother liquor is firstly prepared; the mother liquor is diluted into 100mg / L of a medium-concentration IAA working solution; the above working solution is used to prepare sodium carboxymethylcellulose with the mass concentration of 1%, medium-concentration IAA of sodium alginate with the mass concentration of 8% and glycerogel confining liquid; branches are embedded into a culture medium; and the gel confining liquid is dropwise added onto axillary buds of the robust and thick branches.

Description

technical field [0001] The invention discloses a method for using medium-concentration IAA and glycerin gel at nodes, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as IAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vitro ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N43/38A01P21/00A01G7/06A01G1/00
Inventor 钱夕刚
Owner 钱夕刚