Euglena spp. microalgae, polysaccharide manufacturing method, and organic compound manufacturing method

A manufacturing method and technology of polysaccharides, applied in the direction of microorganism-based methods, microorganisms, microorganisms, etc., can solve the problem that the performance of organic compounds such as polysaccharides is not necessarily sufficient

Active Publication Date: 2016-01-06
KOBELCO ECO-SOLUTIONS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] However, such microalgae of the genus Euglena have a problem that their ability to produce organic compounds such as polysaccharides is not necessarily sufficient.

Method used

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  • Euglena spp. microalgae, polysaccharide manufacturing method, and organic compound manufacturing method
  • Euglena spp. microalgae, polysaccharide manufacturing method, and organic compound manufacturing method
  • Euglena spp. microalgae, polysaccharide manufacturing method, and organic compound manufacturing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0208] 200 mL of the compositions shown in Table 2 were placed in a 500 mL Sakaguchi flask. Furthermore, glucose and yeast extract were added so that the concentration of glucose may be 15 g / L, and the concentration of yeast extract may be 5 g / L. Further, the pH was adjusted to 4.0 by adding hydrochloric acid to prepare a culture solution.

[0209] Then, microalgae of the genus Euglena ( Euglena gracilis ) EOD-1 strain was cultured for 3 days, and the cultivation step was carried out.

Embodiment 2-4

[0211] The culture procedure was carried out in the same manner as in Example 1, except that the amount of glucose was changed so that the glucose concentrations in the culture solution were 20 g / L, 25 g / L, and 30 g / L.

Embodiment 5

[0222] Except for the point of using a 300 ml Erlenmeyer flask; use of a culture solution in which beer was added so that the concentration of ethanol derived from beer became 2.5% by volume relative to the composition shown in 50 mL of Table 2; yeast extract was not added to the culture solution This point; repeating 12 hours of light irradiation environment and 12 hours of dark environment to cultivate this point; the cultivation time is 7 days other than this point, by cultivating microalgae of the genus Euglena ( Euglena gracilis ) EOD-1 strain, the culturing step was carried out. In addition, photosynthetic photon flux density (PPFD) is about 100 μmol / m 2 ·s.

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Abstract

Provided are Euglena spp. microalgae, which are Euglena gracilis Strain EOD-1 (Deposit Number FERM BP-11530) or a variant thereof and have at least a polysaccharide-producing ability. Provided is a polysaccharide manufacturing method for manufacturing polysaccharides by culturing Euglena spp. microalgae, which are Euglena gradilis Strain EOD-1 (Deposit Number FERM BP-11530) or a variant thereof and have at least a polysaccharide-producing ability, as the polysaccharide-producing organism. Provided is an organic compound manufacturing method for manufacturing at least one kind of organic compound selected from a group consisting of polysaccharides, lipids, Vitamin C, Vitamin E, pigments, and proteins by culturing Euglena spp. microalgae, which are Euglena gradilis Strain EOD-1 (Deposit Number FERM BP-11530) or a variant thereof and have at least a polysaccharide-producing ability.

Description

[0001] Cross-references to related applications [0002] This application claims the priority of Japanese Patent Application No. 2013-067558, which is incorporated in the description of the specification of this application by reference. technical field [0003] The present invention relates to microalgae of the genus Euglena, a method for producing polysaccharides, and a method for producing organic compounds. Background technique [0004] Microalgae belonging to the genus Euglena are also called green algae, and are known as microorganisms that produce polysaccharides such as paramylon by culture. [0005] In the past, as the microalgae of the genus Euglena, it is known that there are many kinds, for example, Euglena gracilis ( Euglena gracilis ) NIES-48 strain is known (Patent Document 1). [0006] Such microalgae of the genus Euglena produce polysaccharides such as paramylon by culturing, and accumulate the polysaccharides in cells. [0007] In addition, such microa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P19/04C12P21/00
CPCC12N1/12C12P1/00C12P7/64C12P19/04C12R2001/89C12N1/125C12P7/6436
Inventor 渡边信出村干英河地正伸佐藤夏岐赤司昭竹崎润滨田武志高桥円大开健司
Owner KOBELCO ECO-SOLUTIONS CO LTD
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