Sericin nerve guidance conduit as well as preparation method and application thereof

A technology of sericin and nerve conduit, which is applied in the field of medical biomaterials, can solve the problems of unsatisfactory structure and function recovery, difficult to adjust degradation performance, poor mechanical processing performance, etc., and is beneficial to product quality control and large-scale production. , the effect of promoting migration and alignment, good mechanical properties

Active Publication Date: 2016-01-13
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the performance of this kind of material is unstable, and it is not easy to adjust its degradation performance due to the change of the host enzyme environment, and its mechanical processing performance is poor, so it is difficult to achieve large-scale production; (Using rats as an a

Method used

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  • Sericin nerve guidance conduit as well as preparation method and application thereof
  • Sericin nerve guidance conduit as well as preparation method and application thereof
  • Sericin nerve guidance conduit as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The preparation of embodiment 1 sericin nerve guide

[0063] 1. Selection of silkworm cocoons:

[0064] Silkworm cocoons of silkworm silk fibroin-deficient mutant varieties were selected (purchased from the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, and the silkworm silk fibroin-deficient mutant varieties were preserved in the National Silkworm Resource Preservation Center of the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, preservation number (or commodity number) 185Nd -s, 140Nd-s, 139Nd-s) as raw materials, the main chemical composition is: sericin.

[0065] 2. Extraction and separation of sericin

[0066] Figure 14 It is a schematic flow chart of preparing a sericin nerve guide according to an embodiment of the present invention, and the specific steps are:

[0067] 1) Weigh 1 g of cocoons of silkworm silk fibroin-deficient mutant varieties and cut them into 1 mm 2 Left and right fragments ...

Embodiment 2

[0089] Example 2 Performance analysis of sericin nerve guide

[0090] The following performance analysis is carried out to the sericin nerve guide (sample D1) prepared in embodiment 1, specifically:

[0091] 1. Macro structure

[0092] Using the selected mold, the obtained sericin nerve conduit was prepared into a hollow conduit structure with an inner diameter of 1.5 mm, a wall thickness of 0.75 mm, and a length of 20 mm.

[0093] 2. Microstructure.

[0094] Sericin nerve conduits were observed under a scanning electron microscope (ULTRAPLUS-43-13, Zeiss, Germany). Such as Figure 5 As shown, A and B are SEM images of the cross-section of the sericin nerve conduit, the scales are 500 microns and 100 microns respectively, the interior of the conduit is a multi-void structure with an average pore size of 42.24 μm, which can well support cell growth and promote exchange of nutrients and metabolites; Figure 5 Among them, C and D are the SEM images of the surface of the seri...

Embodiment 3

[0108] Example 3 Sericin Supports Assessment of Nerve Cell Survival and Proliferation Ability

[0109] Experimental group: use the aseptic sericin protein aqueous solution extracted in Example 1 to directly add to the cell well plate, freeze at -80°C for 4 hours, and then freeze-dry in a vacuum freeze dryer to obtain freeze-dried sericin Protein scaffold: Rat Schwann cells (RSC96) collected from cell culture flasks were suspended, blown apart, and planted on cell culture plates covered with freeze-dried sericin scaffolds. The medium used for cell culture was DMEM (Dulbecco's Modified Eagle's Medium) medium, and the cells were placed in a cell culture incubator (37°C, CO 2 The concentration is 5%, and the humidity is 100%).

[0110] In the control group, the cells were directly planted on ordinary well plates.

[0111] Such as Figure 8 As shown, after planting, the CCK-8 method was used to detect the proliferation of cells 1, 3, 5 and 7 days after planting. Depend on Fig...

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Abstract

The invention provides a sericin nerve guidance conduit as well as a preparation method and application thereof. The sericin nerve guidance conduit contains sericin, and the preparation method comprises the following steps: grinding cocoons of fibroin-deleted mutant silkworms which serve as a raw material, sterilizing the ground cocoons by virtue of ultraviolet ray or ethanol, then extracting sericin by virtue of LiBr or LiCl water solution and preparing 0.5-20wt% of a sericin water solution from an extract; adding a covalent cross-linking agent to the sericin water solution, uniformly mixing and molding in a mold so as to obtain a semi-finished sericin nerve guidance conduit product, wherein the dosage of the covalent cross-linking agent is such determined that 2-500ul of the covalent cross-linking agent which is 0.1-50wt% in concentration is added to every 1ml of the sericin water solution; and freeze-drying the semi-finished sericin nerve guidance conduit product, so that the finished sericin nerve guidance conduit is obtained. The sericin nerve guidance conduit provided by the invention is good in bioactivity and biocompatibility; and the sericin nerve guidance conduit is naturally degradable in vivo and is capable of promoting the regeneration of peripheral nerves of long distant defect.

Description

technical field [0001] The invention relates to a sericin nerve guide and its preparation method and application, belonging to the field of medical biomaterials. Background technique [0002] Peripheral nerve injury is a relatively common disease, usually caused by trauma caused by traffic accidents, natural disasters, wars, tumor resection, congenital deformities, etc. About 2.8% of traumatic diseases can lead to peripheral nerve injury, resulting in nerve rupture. Peripheral nerve injury usually leads to sensory and motor dysfunction of patients, high disability rate, seriously affects the quality of life of patients, and brings huge economic burden to families and society. [0003] The principle of treatment for peripheral nerve injury is to restore the continuity of the nerve as early as possible. The current treatment methods mainly include autologous nerve transplantation, allogeneic nerve transplantation, tension-free direct suture of the broken ends, and tissue engin...

Claims

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Application Information

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IPC IPC(8): A61L27/22
Inventor 王琳王征谢洪建杨文罗震王健刘佳
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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