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A kind of enzyme fertilizer and its preparation method and application

A fertilizer and fermentation liquid technology, applied in the field of new high-efficiency compound biological fertilizers, can solve the problem of long fermentation time and other problems, and achieve the effect of purifying and repairing soil, promoting crop growth and development, and promoting growth and development.

Active Publication Date: 2020-08-21
山东宝来利来生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the above-mentioned prior art, since no special bacterial liquid for fermentation is added in the fermentation process, the time for fermenting the material is long, and the production performance of the obtained enzyme fertilizer needs to be further improved.

Method used

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  • A kind of enzyme fertilizer and its preparation method and application
  • A kind of enzyme fertilizer and its preparation method and application
  • A kind of enzyme fertilizer and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Screening of functional strains

[0061] 1 Test materials

[0062] 1.1 Strains

[0063] Shandong Baolai Lilai Biological Engineering Co., Ltd. strain collection room provides 3 strains of lactic acid bacteria (C 2 0038, C 2 0015, C 2 0001), 3 strains of yeast (C 4 0021, C 4 0006, C 4 0030).

[0064] 1.2 Medium

[0065] MRS medium: glucose 2.0%, ammonium citrate 0.2%, sodium acetate 0.5%, dipotassium hydrogen phosphate 0.5%, manganese sulfate 0.02%, magnesium sulfate 0.05%, peptone 1.0%, beef extract 1.0%, yeast extract 0.5%, Tween-800.1%, pH 6.0, both are mass ratios;

[0066] Yeast culture medium: glucose 2.0%, yeast extract 0.5%, peptone 1.0%, dipotassium hydrogen phosphate 0.2%, pH natural, all are mass ratios;

[0067] All reagents used above are of analytical grade.

[0068] 1.3 Reagents and formulations

[0069] 1.3.1 Reagent and formula for determination of amylase content in enzyme fertilizer

[0070] Maltose standard solution 1mg / mL, starch standard solution 2% (m...

Embodiment 2

[0148] Example 2 Preparation of Enzyme Fertilizer

[0149] 1 Test materials

[0150] Huiyuan Juice Factory provides dried pomace and brown sugar with a moisture content of 5%;

[0151] Lactobacillus plantarum LP fermentation broth, the number of viable bacteria is 5.23×10 8 cfu / mL;

[0152] CUM fermentation broth of Candida utilis, the number of viable bacteria is 2.21×10 9 cfu / mL.

[0153] 2 Test method

[0154] 2.1 Preparation method of enzyme fertilizer

[0155] In the early stage of fermentation of the pomace, the main bacteria are yeasts, which carry out aerobic fermentation; the main bacteria in the later stages are lactic acid bacteria. After mixing the pomace, brown sugar, water and the CUM fermentation broth of Candida utilis, sealed and fermented in the fermentation tank, after 30 days of fermentation, supplemented with Lactobacillus plantarum LP fermentation broth, and continued fermentation for 30 days; pomace: brown sugar: water: CUM fermentation broth of Candida utilis: La...

Embodiment 3

[0168] Example 3 Verification of the effect of enzyme fertilizer with the effects of promoting crop growth and improving soil

[0169] 1 Test materials

[0170] The enzyme fertilizer prepared in Example 2 of the present invention;

[0171] Cabbage variety: Liangfeng Siji cabbage on the market;

[0172] 2 Test method

[0173] 2.1 Test grouping:

[0174] A: Blank Control group (the blank group without chemical fertilizers and fertilizers); B: CK-I (single fertilizer ammonium bicarbonate group, press 180kg / hm 2 Applied, diluted 2000 times with water during use); C: CK-Ⅱ (the enzyme fertilizer prepared in Comparative Example 1 of the present invention, at 180kg / hm 2 Applied, diluted 2000 times with water during use); D: CK-Ⅲ (the enzyme fertilizer prepared in Comparative Example 2 of the present invention, at 180kg / hm 2 Applied, diluted 2000 times with water during use); E: EM (the enzyme fertilizer prepared in Example 2 of the present invention, at 180kg / hm 2 Apply, dilute 2000 times with w...

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Abstract

The present invention discloses the application of Candida utilis CUM and Lactobacillus plantarum LP in the preparation of enzyme fertilizers with the effects of promoting crop growth and improving soil, and also discloses the fermented fermented fermented fertilizer by the above-mentioned Candida utilis CUM and Lactobacillus plantarum LP. The enzyme fertilizer made from pomace, and the preparation method of the enzyme fertilizer, the steps are as follows: (1) mix pomace, brown sugar, water and yeast fermentation liquid, and seal fermentation for 25-35 days; Then add the lactic acid bacteria fermentation liquid to the finished material, and seal and ferment for 25-35 days. The invention also discloses the application of the enzyme fertilizer in crop planting. The enzyme fertilizer of the present invention contains a large amount of growth-promoting factors, which can increase the disease resistance function of crops, greatly reduce pesticide residues, and promote the growth and development of crops; it can also purify and restore soil, inhibit soil-borne pathogens, and improve the stress resistance of plants.

Description

Technical field [0001] The invention relates to an enzyme fertilizer and a preparation method and application thereof, and belongs to the technical field of a novel high-efficiency compound biological fertilizer. Background technique [0002] The unreasonable use of industrial fertilizers not only caused a huge waste of resources, but also caused the deterioration of natural ecology and environmental pollution. With the country's demand for ecological agriculture and sustainable development of agriculture, and the rise of global green food production and consumption, the development of green and non-toxic biological fertilizers has become a boom. Biological fertilizer refers to a kind of biological products rich in effective active microorganisms, through the activities of microorganisms to improve the growth environment of crops, nutrient conditions, nutrient conversion, and inhibit the biological activity of harmful microorganisms to exert its specific fertilizer effect. At pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F17/20C05F17/50C05G3/00C05G3/80A01C21/00
CPCY02W30/40
Inventor 单宝龙陈雷谷巍张建梅韩广泉王红侯红燕黄启亮乔蓬蕾陶宁谢全喜于佳民侯楠楠王珂徐辉王春凤
Owner 山东宝来利来生物工程股份有限公司