Bio-fertilizer applicable to cultivation of bletilla striata
A technology of bio-organic fertilizer and bletilla striata, applied in fertilizer mixture, fertilization device, application, etc., can solve the problems of bletilla striata tuber burning, potash loss, root rot, etc., to promote rapid growth, avoid loss, and improve soil structure Effect
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Embodiment 1
[0033] Screening of Bacillus subtilis Lys-979: The high-temperature-resistant Bacillus Lys-979 was isolated from corn stalks, rice straw, and tobacco piles in Yunyuan, Hunan Agricultural University. After morphological identification and molecular identification of 16SRNA evolution tree, the strain Lys- 979 is Bacillus subtilis. Bacillus subtilis Lys-979 was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on September 8, 2015, and the preservation number is CGMCCNo.11333. Bacillus subtilis Lys-979 slant medium formula: yeast extract 0.5%, glucose 2.0%, agar powder 0.7%, pH 7.0, water 250mL, sterilized at 121°C for 25 minutes; cultured at 40°C for 12-18 hours; Bacteria liquid medium formula and fermentation conditions: inoculate one loop of strain Lys-979 test tube slant into four 500mL Erlenmeyer flasks, the medium formula is yeast extract 0.5%, glucose 2.0%, pH7.0, water 250mL, 121°C Sterilize for 25 minutes; ferment at 4...
Embodiment 2
[0035]750kg of cow dung and 250kg of sawdust are mixed, and the water content of the mixture is controlled at 30% to 50%. Inoculate 80L of Bacillus subtilis Lys-979 fermentation broth, mix well and cover with film to keep warm. The temperature of the mixture is controlled above 50° C., and the fermentation time is 8 days. After the fermentation, add 400 kg of dried rapeseed cake and mix to obtain a mixture, then add 2.5% ammonium chloride, 2.5% potassium nitrate, and 1.5% sodium dihydrogen phosphate to the mixture at a final concentration, and stir evenly. Then inoculate 80 kg of Trichosporium cephalospora yeast powder, and after stirring evenly, the water content of the mixture is controlled at 30% to 50%, the temperature is controlled at 20° C. to 30° C., and the stirring and fermentation time is 5 days. After the fermentation of Trichosporium cephalosporin, the fertilizer with yeast smell is directly applied to the field, or it can be granulated into granular fertilizer by...
Embodiment 3
[0041] 1200kg of cow dung and 400kg of sawdust are mixed, and the water content of the mixture is controlled at 30% to 50%. Inoculate 140L of Bacillus subtilis Lys-979 fermentation broth, mix well and cover with film to keep warm. The temperature of the mixture is controlled above 50° C., and the fermentation time is 8 days. After the fermentation, 800 kg of dried rapeseed cake was added and mixed to obtain a mixture, and then 2.5% ammonium chloride, 2.5% potassium nitrate, and 2% sodium dihydrogen phosphate were added to the mixture at a final concentration, and stirred evenly. Then inoculate 170 kg of Trichosporium cephalospora yeast powder, and after stirring evenly, the water content of the mixture is controlled at 30% to 50%, the temperature is controlled at 20° C. to 30° C., and the stirring and fermentation time is 6 days. After the fermentation of Trichosporium cephalosporin, the fertilizer with yeast smell is directly applied to the field, or it can be granulated int...
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