Phellinus linteus mother strain tissue isolation transfer-tube culture method

A technology of tissue separation and cultivation method, which is applied in the field of tissue separation and transfer tube cultivation of Phellinus japonica mother species, can solve the problems of slow germination speed of mycelia, small one-time inoculation amount, high pollution rate, etc., to improve robustness, increase The effect of one inoculation amount and low contamination rate

Inactive Publication Date: 2016-02-03
四川中瑞华航商贸有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the problems of the existing breeding methods of Phellinus japonica, such as too small amount of one-time inoculation, slow mycelium germination speed, high pollution rate, etc., and provide a tissue separation and transfer tube for Phellinus japonica female species that solves the above problems Breeding method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method for cultivating the tissue separation of Phellinus japonica female species by transferring tubes, comprising the following steps:

[0030] (1) Cut the bulk tissue from the Phellinus fruiting body, inoculate the bulk tissue into a test tube culture medium, and cultivate it in a constant temperature dark room until yellow fluffy mycelium grows;

[0031] That is, in the sterilized inoculation box, the surface-sterilized wild Phellinus fruiting body is divided into two halves with both hands, and a small square tissue of about 2*2mm is cut out. Use an inoculation needle to transfer the bacterial flesh tissue to the center of the test tube culture medium. Among them, the utensils and the cut tissue must not be in contact with other parts of the Phellinus thallus to avoid contamination. After inoculation Place it in a constant temperature darkroom at 25°C for cultivation, and after 2 days, yellow fluffy hyphae can be seen growing from the tissue block. At this time...

Embodiment 2

[0039] The difference between this embodiment and embodiment 1 is: in the present embodiment, the components of the test tube culture medium and the expansion medium are different, and the specific composition is as follows:

[0040] 200g peeled potatoes, 20g glucose, KH 2 P0 4 2g, MgSO 4 1g, 1000ml of mulberry juice. The specific production process of mulberry juice is as follows: crush biennial wild mulberry branches with a crusher and pass through a 20-mesh sieve to obtain mulberry branch powder. Weigh 15g of mulberry branch powder and put it into 1000ml water, boil for 15 minutes, and filter it with gauze. Mulberry juice.

[0041] The mother Phellinus japonica produced in this example was stored under the condition of 4-6°C, and the vigor of the Phellinus japonica mother seed was detected respectively at the storage time of 60d, 80d, 100d, and 120d. After testing, the above-mentioned 60d, 80d At 100 d, the mycelium germination speed is even slower, and the mycelium can...

Embodiment 3

[0043] The difference between this embodiment and embodiment 1 is: in the present embodiment, the components of the test tube culture medium and the expansion medium are different, and the specific composition is as follows:

[0044] Peeled potato 190g, glucose 20g, KH 2 P0 4 2g, MgSO 4 1g, VB110mg, mulberry branch powder 15g, water 1000ml. The preparation method of the mulberry branch powder is as follows: the annual wild mulberry branch is dried until the water content is lower than 15%, then crushed with a pulverizer and passed through a 40-mesh sieve to obtain the mulberry branch powder.

[0045] The mother Phellinus japonica produced in this example was stored under the condition of 4-6°C, and the vigor of the Phellinus japonica mother seed was detected respectively at the storage time of 60d, 80d, 100d, and 120d. After testing, the above-mentioned 60d, 80d 、 At 100 days, the Phellinus japonica parent species can germinate mycelium normally; at 120 days, the Phellinus ...

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Abstract

The invention discloses a phellinus linteus mother strain tissue isolation transfer-tube culture method, mainly aiming at solving the problems of an existing phellinus linteus mother strain culture method which is too low in one-time inoculum size, slow in mycelium germination rate, high in pollution rate and the like. The culture method disclosed by the invention comprises the following steps: (1) cutting a massive tissue from a fruiting body of phellinus linteus, inoculating the massive tissue into a test-tube culture medium, and culturing in a constant-temperature darkroom until yellow villous mycelia appear; (2) when the mycelia are 2-3cm away from the massive tissue, picking the culture medium containing edge mycelia, transferring and culturing in another test-tube culture medium, and transferring for more than one time so as to obtain purified mycelia; and (3) as the test-tube culture medium is full of the purified mycelia, dicing the test-tube culture medium full of the purified mycelia, and inoculating and culturing the massive culture medium on an enlarged culture medium so as to obtain a phellinus linteus mother strain. The culture method disclosed by the invention has the advantages of effectively increasing the one-time inoculum size, reducing labor intensity, accelerating a germination rate, reducing a pollution rate and the like.

Description

technical field [0001] The invention relates to a method for cultivating Phellinus japonica female species, in particular to a method for cultivating the Phellinus japonica female species through tissue separation and tube transfer. Background technique [0002] Phellinus, Basidiomycotina, Phyllomycetes, Polyporacea, Polyporaceae, Pinfoliobacteria fungi. Its scientific name is Phellinus Linteus, and its Chinese name is Acupunculus clefthoof, commonly known as Sangchen, Phellinus sphaerifera, etc.; it is a medicinal fungus that parasitizes broad-leaved trees such as mulberry trees, and is known as "forest gold". According to the "Properties of Medicine" record: sweet and pungent, non-toxic. "National Compilation of Chinese Herbal Medicine" records that Phellinus has the effects of "strengthening five viscera, softening firmness, detoxification, hemostasis, promoting blood circulation and stomach diarrhea", and is currently internationally recognized as the most effective med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 顾青月
Owner 四川中瑞华航商贸有限公司
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