A kind of formyl phenylboronic acid modified magnetic nanoparticles and its preparation and application

A formylbenzeneboronic acid and magnetic nanoparticle technology, which is applied in the preparation of test samples, other chemical processes, chemical instruments and methods, etc., can solve problems such as cumbersome synthesis steps, achieve strong enrichment ability, good application prospects, The effect of increasing the amount of bonding

Active Publication Date: 2017-12-05
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the reported boronic acid-modified hybrid magnetic nanomaterials have the disadvantage of cumbersome synthesis steps.

Method used

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  • A kind of formyl phenylboronic acid modified magnetic nanoparticles and its preparation and application
  • A kind of formyl phenylboronic acid modified magnetic nanoparticles and its preparation and application
  • A kind of formyl phenylboronic acid modified magnetic nanoparticles and its preparation and application

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Experimental program
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Effect test

Embodiment 1

[0019] Phenylboronic acid modified magnetic nanoparticles (Fe 3 o 4 @PEI-FPBA) synthesis. The whole synthesis reaction is divided into two steps:

[0020] In the first step, Fe was first synthesized by a one-pot solvothermal reduction method 3 o 4 @PEI Nanoparticles: Use 1.0g ferric chloride hexahydrate as raw material, use 50mL ethylene glycol as the reaction solvent, add 4.0g sodium acetate and 1.0g PEI (weight average molecular weight 70000Da), stir well and move to a 100mL reactor , the reaction temperature is 220°C, the reaction time is 2h, Fe3 o 4 @PEI Magnetic nanoparticles.

[0021] The second step of phenylboronic acid modification: After the material synthesized in the first step was washed alternately with water and ethanol three times each, 0.2 g of the material was dispersed in 300 mL of glacial acetic acid / methanol solution with a volume ratio of 1:124, and 0.2 g of 4 -Formylphenylboronic acid and 0.2g sodium cyanoborohydride react at 40°C for 10h to obtain...

Embodiment 2

[0024] Selective enrichment of catecholamines in urine:

[0025] (1) Urine pretreatment: the urine (containing antioxidants) was thawed, centrifuged at 14,000 rpm / min for 15 min, and ammonia water was added to adjust the pH of the urine to 8.5.

[0026] (2) Enrichment of catecholamines: take 1 mL of urine adjusted to alkaline, add 8 mg of the phenylboronic acid-modified magnetic nanomaterial Fe in Example 1 3 o 4 @PEI-FPBA, ultrasonically disperse for 1 min, then vortex for 2 min. After the magnetic material is separated by a magnet, the supernatant is removed, the material is washed three times with a pH 8.5 ammonia solution (containing 50% acetonitrile), and then 1 mL of a pH 3.5 formic acid solution (containing 80% acetonitrile) is added for elution, ultrasonication for 1 min, and vortex 1min. The magnetic nanomaterials were removed by magnetic separation, and 800 μL of the eluent was taken to freeze-dry, and then reconstituted with 40 μL of reconstitution solution, and ...

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Abstract

The invention belongs to the field of inorganic materials and analysis technology, and specifically relates to a simple preparation method of formylphenylboronic acid-modified magnetic nanoparticles (Fe3O4@PEI-FPBA) and its application in the selective enrichment of catecholamines in urine. The magnetic microspheres are synthesized by a one-pot method of Fe3O4@PEI, and then grafted with phenylboronic acid functional groups through reductive amination reaction to form hybrid magnetic nanoparticles. The magnetic material has the characteristics of simple preparation method, large adsorption capacity, good selectivity, and short equilibration time, and the pretreatment process is simple, fast, and strong in specificity when selectively enriching trace catecholamines in urine. The material has excellent application prospects in the enrichment of 1,2-cis-dihydroxy compounds in body fluids.

Description

technical field [0001] The invention belongs to the field of inorganic materials and analysis technology, and specifically relates to a formylphenylboronic acid-modified magnetic nanoparticle (Fe 3 o 4 @PEI-FPBA) simple preparation method and its application. [0002] The magnetic nanoparticles designed and proposed by the invention have a simple preparation process and can be used as an affinity probe to achieve highly selective separation and enrichment of trace 1,2-cis-dihydroxy compounds in complex biological samples. The material has good application prospects in the field of metabolomics. Background technique [0003] Catecholamines mainly include dopamine (DA), epinephrine (EP), norepinephrine (NE) and isoproterenol (IP). The chemical structures of these substances have a common feature: they all have a dihydroxybenzene nucleus and a side chain with an amino group. This type of substance is an endogenous active substance of organisms, has strong biological effects...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/286B01J20/30B01D15/38G01N1/40
Inventor 许国旺秦倩石先哲李华
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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