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Method and device for accurate pipette discharge volume calibration

A calibration method and discharge capacity technology, which is applied in the field of pipettes, can solve problems such as difficulty in volume discharge, difficulty in ensuring accuracy and reliability, etc., and achieve the effect of simple calibration method and simple calibration

Inactive Publication Date: 2016-02-10
A&D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In such a field, since the conditions are different between when the suction pipe is calibrated with distilled water and when it is used, it is difficult to discharge the correct volume, and of course it is also difficult to ensure accuracy and reliability.

Method used

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  • Method and device for accurate pipette discharge volume calibration
  • Method and device for accurate pipette discharge volume calibration
  • Method and device for accurate pipette discharge volume calibration

Examples

Experimental program
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Embodiment Construction

[0027] Next, preferred embodiments of the present invention will be described with reference to the accompanying drawings. figure 1 is a front view of an electric pipette showing an embodiment of the present invention, figure 2 This is the right side view of the electric pipette.

[0028]Reference numeral 100 is an electric pipette, which is a micropipette operated by hand having a total length of about 280 mm. Reference numeral 1 is a cylindrical main body case 1 that is long in the longitudinal direction. Inside the case, which is formed by fitting a front case and a rear case, an elongated cylindrical body 2 for suction and discharge of liquid is provided. A piston 3 inserted into the cylindrical body 2 so as to reciprocate in the vertical direction, a ball screw 5 connected to the piston 3 and moving the piston 3 in the vertical direction, and a ball screw 5 for rotating the ball screw 5 in both forward and reverse directions Driven stepper motor 6.

[0029] Reference ...

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PUM

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Abstract

Provided is a simple pipette discharge volume calibration method, and corresponding device, which can secure the correct discharge amount under any measurement conditions. In order to achieve that the volume actually discharged is the configured amount, this method involves a step (S1) for configuring a configured amount with the pipette that actually does the measuring, a step (S2) for sucking the liquid to be measured into the tip that where the measuring is performed, a step (S3) for discharging the liquid sucked into the tip into a mass measuring device that can measure weight, steps (S4-S6) for verifying the actual amount actually discharged from the pipette, and steps (S7-S9) for calculating the ratio of the configured volume to the actual volume and calibrating to suck in the amount obtained by multiplying the configured amount by said ratio.

Description

technical field [0001] The present invention relates to a pipette as a measuring device that sucks and discharges a liquid of a set volume, and particularly relates to a method for accurately correcting the discharge capacity of the pipette and a pipette device used for the pipette. Background technique [0002] A metering device called a pipette with a constant volume discharge function of liquid grasps the volume change caused by the movement of the movable piston equipped inside the pipette as the volume change of the air, and calculates the volume change of the air. The volume of liquid is sucked and discharged to the tip mounted on the tip of the pipette. In principle, since compressible air is used as the medium, the air circuit receives resistance from air, or is subjected to piston operation, tip (full length of tip, tip shape of tip, etc.), and physical properties of the liquid to be sucked. (hydrophobicity, hydrophilicity, hygroscopicity, etc.), or various influen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/02G01F13/00G01F25/00G01N1/00
CPCG01N1/00G01F13/00B01L3/021B01L2200/148B01L2300/0645G01F25/0084G01G1/00G01G23/01G01F11/022G01F11/023G01F11/027B01L3/0227B01L3/0237B01L2300/0627B01L2300/0832
Inventor 出云直人深见雄二
Owner A&D CO LTD