Long-term-subculture butterfly orchid protocrom-like body aging alleviating method

A technology similar to protocorms and Phalaenopsis, applied in the field of horticultural plant biology, can solve the problems of cumbersome operation, complicated medium formula, and unclear long-term subculture preservation effect, and achieve the effect of slowing down proliferation and easy operation

Active Publication Date: 2016-02-17
SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are not many reports on the subculture maintenance methods of plant cultures, almost all of which use callus or somatic embryos as materials. The above-mentioned application medium formula is complicated, or the operation is cumbersome, and the effect of long-term subculture preservation at room temperature is not clear

Method used

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  • Long-term-subculture butterfly orchid protocrom-like body aging alleviating method
  • Long-term-subculture butterfly orchid protocrom-like body aging alleviating method
  • Long-term-subculture butterfly orchid protocrom-like body aging alleviating method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The protocorm-like stems of the first generation (0 months) of Phalaenopsis were inoculated on the subculture medium 1 / 2MS + coconut milk 20% (v / v) + sucrose 2% (w / v). The treatment group was subculture medium supplemented with antioxidant cysteine ​​100mg / L medium, DNA methylation inhibitor mannitol 8g / L medium, mannitol was added to the medium for direct autoclaving, and cysteine ​​mixed first Soluble in water, filter and sterilize, pour into the uncondensed medium that has been autoclaved, divide into culture bottles, and inoculate protocorms of Phalaenopsis after condensation. The culture conditions are all light 1500lx, 12h / d, temperature 24-26°C. At the 24th month of culture (12 times of multiplication in bottles during the period), the proliferation ability of protocorms (fresh weight in each bottle), survival rate and differentiation rate were tested, see Table 1.

[0013] Table 1. Fresh weight, survival rate and germination rate of subcultured protocorms for 2...

Embodiment 2

[0021]The protocorm-like plants of the first generation (0 months) of Phalaenopsis were inoculated in the subculture medium 1 / 2MS+6-BA3mg / L+coconut juice 20% (v / v)+sucrose 2% (w / v). The treatment group added antioxidant cysteine ​​100mg / L medium and DNA structure stabilizer spermine 40mg / L medium for the subculture medium. The two were first miscible in water, filtered and sterilized and injected into the high-pressure sterilized non-condensed medium. The culture medium is divided into culture bottles and inoculated with Phalaenopsis protocorms after condensation. The culture conditions are all light 1500lx, 12h / d, temperature 24-26°C. At the 24th month of culture (12 times of multiplication in bottles during the period), the proliferative ability (fresh weight of each bottle), survival rate and differentiation rate of protocorms were tested, see Table 3.

[0022] Table 3. Fresh weight, survival rate and germination rate of protocorm-like subculture for 24 months

[0023] ...

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Abstract

A long-term-subculture butterfly orchid protocrom-like body aging alleviating method is as follows: an antioxidant, a DNA methylation inhibitor and a DNA structure stabilizer are added into a subculture medium for alleviating protocrom-like body aging and maintaining genetic material DNA stable, and the three types of regulators can be mixed simultaneously, can also be pairwise combined for use. The antioxidant mainly comprises ascorbic acid, cysteine salt, glutathione, sodium bisulfite, sodium selenite and thiourea. The DNA methylation inhibitor mainly comprises 5-aza-cytidine, betaine, choline, tea polyphenols and mannitol. The DNA structure stabilizer mainly comprises spermine, spermidine and putrescine. The long-term-subculture butterfly orchid protocrom-like body aging alleviating method can slow down long-term-subculture butterfly orchid protocrom-like body proliferation and decrease differentiation capacity, has certain repair effects on certain physiological and molecular markers of protocrom-like body, and is in no need of use of special equipment and easy to operate. The used chemical regulators have no toxic side effects on plant materials.

Description

technical field [0001] The invention belongs to the biotechnology of horticultural plants in the field of agriculture and forestry, and specifically relates to a method for slowing down the aging of protocorms of Phalaenopsis for long-term succession by using a chemical method. Background technique [0002] In the famous orchid industry, only Phalaenopsis (Moth Orchid) has truly formed professional, large-scale and commercial production. Phalaenopsis ), Phalaenopsis is also the most important New Year’s flower in my country. Tissue cultured Phalaenopsis Protobulb (PLB) has considerable application potential in rapid seedling cultivation, gene transformation, artificial seed development, and germplasm preservation. Although protocorm-like multiplication coefficient is large, in the production process, in order to improve production efficiency (expand the multiplication rate) and be affected by various factors such as market demand and supply time, frequent subculture will ine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 刘福平
Owner SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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