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Preparation method of targeted liposome-encapsulated aqueous phase gold nanocomposite

A technology for targeting liposomes and gold nanoparticles, which can be used in liposome delivery, medical preparations with non-active ingredients, non-active ingredients of polymer compounds, etc., which can solve the problems of easy allergy, nephrotoxicity, short development time, etc. , to achieve the effect of simple process, satisfying production and application, and stable properties

Active Publication Date: 2018-02-09
智玺那诺(上海)生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the most widely used CT iodine contrast agent has the disadvantages of being easily cleared by the kidneys, resulting in short developing time, easy allergies, and certain nephrotoxicity.

Method used

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  • Preparation method of targeted liposome-encapsulated aqueous phase gold nanocomposite
  • Preparation method of targeted liposome-encapsulated aqueous phase gold nanocomposite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Preparation of targeted liposomes:

[0023] Dissolve sulfhydryl-modified polypeptide molecule SH-N1, 2.15mg in 1ml phosphate buffer solution (PBS, pH=7.0), maleimide-modified phospholipid molecule (DSPE-PEG-MAL) 8.9mg in 0.5ml N,N-Dimethylformamide (DMF). The DSPE-PEG-MAL solution was then added to the peptide solution. Bubbles were removed from the solution by sonication, followed by magnetic stirring for 1 hour.

[0024] Dilute the reaction solution to 5ml, put it into a dialysis bag for dialysis, and change the water every 1-2 hours to remove DMF, PBS, and unlinked polypeptide molecules. A phospholipid molecule (P-DSPE) linked to a polypeptide (P) is obtained. Freeze drying gave a solid product.

[0025] Mix 8 mg of hydrogenated soybean phospholipid (HSPC), 3.4 mg of cholesterol, and 1 mg of P-DSPE, add 2.5 ml of chloroform to dissolve in a round bottom flask, and then rotary evaporate at 41 ° C until a film is formed on the wall of the bottle. Place in a vac...

Embodiment 2

[0029] 1. Preparation of targeted liposomes

[0030] Dissolve 2.15 mg of sulfhydryl-modified peptide molecule SH-N2 targeting neuroflagellin in 1 ml of phosphate buffer solution (PBS, pH=7.0), maleimide-modified phospholipid molecule (DSPE- PEG-MAL) 8.9mg was dissolved in 0.5ml N,N-dimethylformamide (DMF). The DSPE-PEG-MAL solution was then added to the peptide solution. Bubbles were removed from the solution by sonication, followed by magnetic stirring for 1 hour.

[0031] Dilute the reaction solution to 5ml, put it into a dialysis bag for dialysis, and change the water every 1-2 hours to remove DMF, PBS, and unlinked polypeptide molecules. A phospholipid molecule (P-DSPE) linked to a polypeptide (P) is obtained. Freeze drying gave a solid product.

[0032] Mix 8 mg of hydrogenated soybean phospholipid (HSPC), 3.4 mg of cholesterol, and 1 mg of P-DSPE, add 2.5 ml of chloroform to dissolve in a round bottom flask, and then rotary evaporate at 41 ° C until a film is formed ...

Embodiment 3

[0036] 1. Preparation of targeted liposomes

[0037] Dissolve Toll-like receptor 4 antibody, 20ml (1mg / ml) in 1ml phosphate buffer solution (PBS, pH=7.0), 8.9mg of maleimide-modified phospholipid molecules (DSPE-PEG-MAL) in 0.5ml N,N-Dimethylformamide (DMF). The DSPE-PEG-MAL solution was then added to the peptide solution. Bubbles were removed from the solution by sonication, followed by magnetic stirring for 1 hour.

[0038] Dilute the reaction solution to 5ml, put it into a dialysis bag for dialysis, and change the water every 1-2 hours to remove DMF, PBS, and unlinked polypeptide molecules. A phospholipid molecule (P-DSPE) linked to a polypeptide (P) is obtained. Freeze drying gave a solid product.

[0039] Mix 8 mg of hydrogenated soybean phospholipid (HSPC), 3.4 mg of cholesterol, and 1 mg of P-DSPE, add 2.5 ml of chloroform to dissolve in a round bottom flask, and then rotary evaporate at 41 ° C until a film is formed on the wall of the bottle. Place in a vacuum ove...

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Abstract

The invention relates to a preparation method of a compound with aqueous-phase nanogold wrapped with targeted liposomes. The preparation method includes the steps of preparing targeted liposomes and the step of wrapping gold nanoparticles with targeted liposomes to obtain microspheres with nanogold wrapped with liposomes. According to the preparation method, the structure with the gold nanoparticles wrapped with targeted liposomes is adopted; adopted raw materials are high in biosecurity, part of the raw materials form commodities already, and therefore certain application prospects are achieved; the prepared composite gold nanoparticles wrapped with targeted liposomes have nature stability, targeting performance and the angiography function. The preparation method is simple in process, high in operability and capable of further meeting production and application requirements.

Description

technical field [0001] The invention relates to a preparation method of targeting liposome-encapsulated aqueous phase nano-gold complex. In particular, it relates to a preparation method for liposome-wrapped aqueous-phase gold nanoparticles linked by targeting polypeptides. The invention has application prospects in the fields of tumor imaging, hyperthermia and drug loading. Background technique [0002] Early diagnosis of tumors urgently needs a biological probe with multiple mode combination, high efficiency and specific active targeting. Computerized tomography (CT) is currently the most widely used diagnostic method, with high spatial resolution, and with the application and development of multi-slice spiral CT, the imaging is getting closer to the natural anatomical structure. However, due to the lack of natural contrast between certain organ structures and surrounding tissues, substances with a density higher or lower than that of the organ need to be introduced into...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K41/00A61K49/04A61K47/64A61K47/68A61P35/00A61K47/69
CPCA61K9/1278A61K41/0052A61K47/42A61K49/0466
Inventor 何丹农王萍刘婷金彩虹吴颖为陶晓峰
Owner 智玺那诺(上海)生物科技有限责任公司