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Method for improving iron-deficiency resistance of rice in seedling stage

A technology of rice seedlings and rice, applied in the field of improving iron deficiency tolerance of rice seedlings, can solve problems such as iron deficiency, iron chlorosis, etc., and achieve the effect of solving iron utilization and high tolerance

Active Publication Date: 2016-03-02
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

Although the enhanced synergistic expression of these genes under iron deficiency stress may improve and promote the absorption of iron by rice, however, since most of the iron in the soil exists in an insoluble state, and a large amount of plant ash is applied on the alkaline soil or the seedling board Alkaline fertilizers such as lime and lime can cause ferrous ions in the soil to react with alkaline substances and precipitate; in addition, during the seedling stage of semi-arid seedling raising in sandy soil, ferrous ions are oxidized to high-priced iron ions that cannot be absorbed by the seedlings. It is easy to cause the seedlings to fail to absorb enough iron and appear symptoms of iron deficiency and chlorosis

Method used

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  • Method for improving iron-deficiency resistance of rice in seedling stage
  • Method for improving iron-deficiency resistance of rice in seedling stage
  • Method for improving iron-deficiency resistance of rice in seedling stage

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Embodiment

[0026] The invention discloses a method for improving iron deficiency tolerance of rice seedlings, which enhances the iron deficiency tolerance of rice by introducing the rice OsRSI gene into the target rice.

[0027] Specific steps are as follows:

[0028] (1) Isolation and cloning of rice OsRSI gene

[0029] According to the complete rice gene sequence and the coding sequence of the OsRSI gene published on the rice database website (http: / / rice.plantbiology.msu.edu / index.shtml), the primer sequences were designed, and enzymes were added to the 5' ends of the two primers. Sequence of cutting sites and protection bases. The primer sequences were: pE-F: 5'-cgggtaccATGCCCGTGACTCAGCACTTAG-3' (SEQ ID NO.2); pE-R: 5'-cgggatccTCACGCTTCGCGTCCCTGGTC-3' (SEQ ID NO.3). 取缺铁处理(缺铁处理方法参见ChengLJ等MutationinnicotianamineaminotransferasestimulatedtheFe(II)acquisitionsystemandledtoironaccumulationinrice.PlantPhysiology2007,145:1647-1657;ZhengLQ等Physiologicalandtranscriptomeanalysisofironandphos...

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Abstract

The invention discloses a method for improving iron-deficiency resistance of rice in a seedling stage. A recombinant vector containing constitutive promoters and OsRSI genes is used for rice transformation, and obtained transgenic rice has high tolerance in an iron deficiency state in the seedling stage. According to the method for improving the iron-deficiency resistance of the rice in the seedling stage, the technical problem of iron utilization in the rice in the iron-deficiency state in the rice seedling stage can be effectively solved.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for improving iron deficiency resistance of rice seedlings. Background technique [0002] Iron is an essential nutrient element for plants, and plays an extremely important role in the electron transport chain or enzymatic reactions of many physiological metabolic processes such as photosynthesis, respiration, protein and nucleic acid synthesis in plants. At the same time, iron, as the active center or prosthetic group of many important enzymes and proteins, participates in the most basic biochemical reactions in life activities. [0003] The total iron content in the soil is high, but the available iron content that can be absorbed and utilized by plants is very low. In agricultural production, due to the insufficient content of soluble iron that can be absorbed and utilized in the soil, iron deficiency chlorosis occurs in plants, which leads to large-scale produ...

Claims

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Application Information

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IPC IPC(8): C12N15/84C12N1/21A01H5/00
Inventor 易可可王芳邓敏娟徐磊赵红玉
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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