A method for improving iron deficiency tolerance in rice seedling stage
A technology of rice seedlings and rice, applied in the field of improving iron deficiency tolerance of rice seedlings, can solve the problems of iron deficiency, iron chlorosis, etc., and achieve the effect of solving iron utilization and high tolerance
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[0026] The invention discloses a method for improving iron deficiency tolerance of rice seedlings, which enhances the iron deficiency tolerance of rice by introducing the rice OsRSI gene into the target rice.
[0027] Specific steps are as follows:
[0028] (1) Isolation and cloning of rice OsRSI gene
[0029] According to the complete rice gene sequence and the coding sequence of the OsRSI gene published on the rice database website (http: / / rice.plantbiology.msu.edu / index.shtml), the primer sequences were designed, and enzymes were added to the 5' ends of the two primers. Sequence of cutting sites and protection bases. The primer sequences were: pE-F: 5'-cgggtaccATGCCCGTGACTCAGCACTTAG-3' (SEQ ID NO.2); pE-R: 5'-cgggatccTCACGCTTCGCGTCCCTGGTC-3' (SEQ ID NO.3). Take iron deficiency treatment (see Cheng LJ et al. Mutation in nicotianamine aminotransferase stimulated the Fe(II) acquisition system and led to iron accumulation inrice. Plant Physiology 2007,145:1647-1657; Zheng LQ ...
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