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Preparation method for three phenolic acid monomeric compounds in salvia prattii hemsl.

A technology of Kangding sage and phenolic acids, which is applied in the field of preparation of three phenolic acid monomer compounds in Kangding sage, can solve the problems of long time consumption, a large amount of organic solvent, and low recovery rate, and achieve Reduce the consumption of organic solvents and energy consumption, the consumption of organic reagents is small, and the effect of low cost

Inactive Publication Date: 2016-03-09
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many traditional methods, including silica gel and gel filtration chromatography, take a long time to prepare, separate and purify phenolic acids, and require a large amount of organic solvents and multi-step separations, resulting in low recovery and high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation method of three kinds of phenolic acid monomer compounds in Sage Kangding of embodiment 1 comprises the following steps:

[0019] (1) Pulverize the root of the crushed sage sage, use alcohol with a mass concentration of 70% to carry out hot reflux extraction at 80° C., extract 3 times, each time for 2 hours, and combine to obtain the extract;

[0020] (2) Evaporate the extract to constant weight under reduced pressure at a temperature of 70° C. and a pressure of 0.07 MPa to obtain the Sage Kangding extract;

[0021] (3) Dissolving the Sage Kangding extract with deionized water first, then extracting 3 times with sherwood oil, ethyl acetate, n-butanol successively, to obtain sherwood oil, ethyl acetate, n-butanol extract and Four parts of the residual aqueous solution; four parts of the extract were evaporated to constant weight under reduced pressure at a temperature of 70°C and a pressure of 0.06 MPa to obtain four parts of the extract;

[0022] (4) Di...

Embodiment 2

[0025] The preparation method of three kinds of phenolic monomer compounds in Sage Kangding of embodiment 2 comprises the following steps:

[0026] (1) Pulverize the root of the crushed Sage sage, use alcohol with a mass concentration of 50% to carry out hot reflux extraction at 70°C, extract 4 times, each time for 3 hours, and combine to obtain the extract;

[0027] (2) Evaporate the extract to constant weight under reduced pressure at a temperature of 60° C. and a pressure of 0.08 MPa to obtain the Sage Kangding extract;

[0028] (3) Dissolving the Sage Kangding extract with deionized water first, then extracting 5 times with sherwood oil, ethyl acetate, n-butanol successively, to obtain sherwood oil, ethyl acetate, n-butanol extract and Four parts of the residual aqueous solution; four parts of the extract were evaporated to constant weight under reduced pressure at a temperature of 70°C and a pressure of 0.07 MPa to obtain four parts of the extract;

[0029] (4) Dissolvin...

Embodiment 3

[0032] The preparation method of three kinds of phenolic acid monomer compounds in Sage Kangding of embodiment 3 comprises the following steps:

[0033] (1) Select the root of Sage Kangding and pulverize it, use alcohol with a mass concentration of 60% to carry out hot reflux extraction at 60°C, extract 4 times, each time for 2 hours, and combine to obtain the extract;

[0034] (2) Evaporate the extract to constant weight under reduced pressure at a temperature of 65°C and a pressure of 0.06 MPa to obtain the Sage Kangding extract;

[0035] (3) Dissolving the Sage Kangding extract with deionized water first, then extracting 3 times with sherwood oil, ethyl acetate, n-butanol successively, to obtain sherwood oil, ethyl acetate, n-butanol extract and Four parts of the residual aqueous solution; four parts of the extract were evaporated to constant weight under reduced pressure at a temperature of 60°C and a pressure of 0.07 MPa to obtain four parts of the extract;

[0036] (4) ...

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Abstract

The invention relates to a preparation method for three phenolic acid monomeric compounds in salvia prattii hemsl. The method comprises the steps of: (1) performing heat reflux extraction on the crushed roots of salvia prattii hemsl. with alcohol and combining the mixture to obtain an extraction liquid; (2) evaporating the extraction liquid at a reduced pressure to dryness to a constant weight to obtain salvia prattii hemsl.extract; (3) after dissolving the salvia prattii hemsl. extract with deionized water, extracting the extract by using petroleum ether, ethyl acetate and n-butanol sequentially to obtain four parts of extract; (4) dissolving the n-butanol extract in water and eluting the n-butanol extract by a D101 macroporous resin column to obtain an eluant containing phenolic acid substances; (6) evaporating the eluant to dryness to a constant weight to obtain coarse phenolic acid extract; and (7) separating the coarse phenolic acid extract in a n-hexane-ethyl acetate-methanol-water system by adopting high-speed counter-current chromatographic equipment to separately obtain monomeric compounds of three active phenolic acid substances: caffeic acid, rosmarinic acid and rosmarinic acid. The preparation method provided by the invention is simple and convenient, fast, effect and low in cost.

Description

1. Technical field [0001] The invention belongs to the field of traditional Chinese medicine phytochemistry, and in particular relates to a preparation method of three kinds of phenolic acid monomer compounds in Sage Kangding. 2. Background technology [0002] Kangding sage (SalivaprattiiHemsl.) belongs to Labiatae (Labiatae) Salvia (SalviaLinn.) plants. The genus Salvia (Salvia L.) is an important group of medicinal plants, and many plants in this genus became important medicinal plants as early as thousands of years ago. Existing data show that: Salvia genus plants contain a variety of useful secondary metabolites, such as: phenolic acids, terpenes, flavonoids and other chemical components. These ingredients have a wide range of physiological and pharmacological activities, including dilating coronary arteries, increasing coronary flow, improving microcirculation, protecting the heart, anti-platelet aggregation, improving immunity, improving the body's ability to withstan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/42C07C59/52C07C67/48C07C69/732C07H15/203C07H1/08
CPCC07C51/42C07C67/48C07H1/08C07H15/203C07C59/52C07C69/732
Inventor 迟晓峰胡风祖陈世龙董琦肖远灿
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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