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A method for repeatedly extracting leech saliva

A technology of repeated extraction and leech, which is applied in the preparation method of peptides, from leech inhibitors, chemical instruments and methods, etc., can solve the problems such as the inability to improve the disease resistance of leech, save costs, reduce prices, and solve low-efficiency problems. Effect

Inactive Publication Date: 2018-12-21
汤浩强
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a method for repeated extraction of leech saliva, aiming to solve the problem in the prior art that direct feeding with blood cannot improve the disease resistance of leeches

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Fish out mature and healthy leeches from the rearing pond and place them in a clear water pond. Change the water and wash them every 2 days to remove impurities on the surface of the living leeches. They will not be fed for one month. One month later, the living leeches were washed again with clear water. At room temperature, the leeches were placed in a storage tank filled with blood, and the intestines filled with blood were put into the storage tank. The leeches sucked the blood in the intestines and were fully fed. Instantly raise the temperature of the storage tank to 50°C, and collect the saliva spit out by the leeches. Instantly raise the temperature 3 times, and collect the saliva spit out by the leech.

[0030] After testing, the hirudin content in these saliva was 45AT-(U international unit) / mL.

Embodiment 2

[0032] Fish out mature and healthy leeches from the rearing pond and place them in a clear water pond. Change the water and wash them every 2 days to remove impurities on the surface of the living leeches. They will not be fed for one month. One month later, the living leeches were washed again with clear water. At room temperature, the leeches were placed in a storage tank filled with blood, and the intestines filled with blood were put into the storage tank. The leeches sucked the blood in the intestines and were fully fed. The temperature of the storage tank was slowly raised to 70°C, and the saliva spit out by the leeches was collected. Instantly raise the temperature 3 times, and collect the saliva spit out by the leeches.

[0033] After testing, the hirudin content in these saliva was 50AT-(U international unit) / mL.

Embodiment 3

[0035] Fish out mature and healthy leeches from the rearing pond and place them in a clear water pond. Change the water and wash them every 2 days to remove impurities on the surface of the living leeches. They will not be fed for one month. One month later, the living leeches were washed again with clear water. At room temperature, the leeches were placed in a storage tank filled with blood, and the intestines filled with blood were put into the storage tank. The leeches sucked the blood in the intestines and were fully fed. Instantly raise the temperature of the storage tank to 40°C, and collect the saliva spit out by the leeches. Instantly raise the temperature 3 times, and collect the saliva spit out by the leech.

[0036] After testing, the hirudin content in these saliva was 52AT-(U international unit) / mL.

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Abstract

The invention discloses a method for leech saliva repeated extraction. The method comprises the following steps: glutamic acid, arginine and sodium chloride are added in fresh blood, and the mixture is mixed uniformly; fresh animal intestines are selected, loaded with the blood and tied up; leeches are placed near intestines loaded with the blood and take in the blood in the intestines; after the leeches are full completely, the ambient temperature is changed, thus the leeches spit saliva, and the leech saliva is collected and obtained. The method can achieve continuous extraction for multiple times, the cost is saved, the hirudin efficacy is raised, leech living bodies are utilized repeatedly, the problems of low efficiency, complex technology and high cost for hirudin extraction one-time utilization at present are solved, and the method facilitates to save and protect the wild leech resource of our country and facilitates to lower the medicine hirudin raw material price greatly.

Description

technical field [0001] The invention relates to the field of hirudin extraction, in particular to a method for repeated extraction of leech saliva. Background technique [0002] Leeches, commonly known as leeches, are a kind of precious Chinese herbal medicines, which are recorded in many medical classics in ancient my country. It is recorded in the "Compendium of Materia Medica" of the Ming Dynasty: water frogs "mainly drive away bad blood, trace blood, moon closure, and accumulate under broken blood. Childless, Lishuitong...heals wounds, falls, and animal blood." Modern medical research shows that leech salivary glands contain an anti-coagulant substance—hirudin, which can be widely used in medical fields such as replantation of severed limbs and inhibition of cancer cell growth. With the application and improvement of modern biotechnology, the extraction and processing of hirudin and the clinical application of medicine, as well as the development, development and promoti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/815C07K1/14
CPCC07K14/815
Inventor 汤浩强
Owner 汤浩强
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