Application of C21orf62 gene in liver cancer diagnosis

A gene, liver cancer technology, applied to the C21orf62 gene in the field of liver cancer diagnosis

Inactive Publication Date: 2016-03-30
陈娟 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many genes related to liver cancer have been reported, most of these genes are only "by-products" caused by the dysregulated expression of other cancer-related genes, and are not really related to the occurrence of cancer

Method used

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  • Application of C21orf62 gene in liver cancer diagnosis
  • Application of C21orf62 gene in liver cancer diagnosis
  • Application of C21orf62 gene in liver cancer diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Screening Gene Markers Related to Liver Cancer

[0051] 1. Sample collection

[0052] 8 cases of normal tissue and liver cancer tissue samples were collected respectively. All the above samples were obtained with the consent of the organizational ethics committee, and the samples were collected and frozen in liquid nitrogen.

[0053] 2. Preparation of RNA samples (operated using QIAGEN tissue RNA extraction kit)

[0054] 1) Sample pretreatment: Take the sample from the -80°C liquid nitrogen tank and put it into a homogenizer soaked in 0.1% DEPC water overnight and sterilized, and grind it into powder; the sample must always be kept in the liquid during the grinding process. In a nitrogen environment, the grinding cup must be pre-cooled, and liquid nitrogen is added while grinding.

[0055] 2) Cleavage: add 1ml Trizol RNA extraction solution to the homogenizer, homogenize at room temperature for 10 minutes, and let stand for 10 minutes to fully lyse the nucle...

Embodiment 2

[0074] Example 2 QPCR sequencing to verify the differential expression of C21orf62 gene

[0075] 1. Large-sample QPCR verification of differential expression of C21orf62 gene. According to the sample collection method in Example 1, 60 cases of liver cancer tissues and 60 normal tissues were selected.

[0076] 2. The specific operation steps of QPCR are as follows:

[0077] (1) RNA extraction

[0078] After collecting the samples, freeze them in liquid nitrogen. After taking them out, put the tissue into a pre-cooled mortar for grinding. After the tissue sample is powdered:

[0079]1) Add Trizol and place at room temperature for 5 minutes;

[0080] 2) Add 0.2ml of chloroform, vibrate the centrifuge tube vigorously, mix well, and place it at room temperature for 5-10min;

[0081] 3) Centrifuge at 12000rpm for 15min, transfer the upper aqueous phase to another new centrifuge tube (be careful not to absorb the protein material between the two aqueous phases), add an equal volu...

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Abstract

The invention discloses application of a C21orf62 gene in liver cancer diagnosis. The C21orf62 gene is in low expression in tissues of a liver cancer patient, through detecting the expression level of the C21orf62 gene, whether a patient suffers from liver cancer can be diagnosed, and through the application of the C21orf62 gene in liver cancer diagnosis, sensitivity and specificity of liver cancer diagnosis are greatly improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of C21orf62 gene in the diagnosis of liver cancer. Background technique [0002] Liver cancer is a malignant tumor of the liver, which can be divided into two categories: primary and secondary. Primary malignant tumors of the liver originate from the epithelial or mesenchymal tissues of the liver. The former is called primary liver cancer, which is a malignant tumor with a high incidence in my country and is extremely harmful; rare. Secondary or metastatic liver cancer refers to malignant tumors originating from multiple organs throughout the body and invading the liver. Liver cancer is one of the most common and most malignant cancers, which occurs frequently in Asia and Africa. In recent years, the incidence of liver cancer in western countries has gradually increased. At present, liver cancer has become the second largest cancer killer in my country, with a high ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/574
CPCC12Q1/6886C12Q2600/158G01N33/57438G01N33/57484
Inventor 陈娟李风玲
Owner 陈娟
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