A Method for Detection and Compensation of Coordinate and Displacement Errors of Microscopic Operating System
A technology of displacement error and operating system, which is applied in the direction of measuring devices, instruments, optical devices, etc., to achieve the effect of simple operation, wide application range and strong versatility
Active Publication Date: 2017-12-05
SOOCHOW UNIV AFFILIATED CHILDRENS HOSPITAL
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However, there is still a displacement error during the movement process. How to calculate the displacement error and make reasonable compensation to make the micromanipulation more accurate is based on the further technical problems to be solved after the above application
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[0053] Below in conjunction with the embodiment shown in the accompanying drawings, the present invention is described in detail as follows:
[0054] First, obtain the deflection angle and displacement error between the screen coordinate system and the platform coordinate system through the following steps:
[0055] (1) First capture the first picture through the CCD camera. In this embodiment, set the length and width of the screen to 640PX*480PX (determined by the imaging resolution of the CCD), and set the top left corner of the first picture to be A , send an instruction to the platform controller to move 480PX to the right along the X direction (3 / 4 of the screen length). After the platform moves, capture the second picture through the CCD, and set the top left corner of the second picture as A 1 ;
[0056] (2) Since the fixed length of each image is 640PX, there must be overlap between the captured first image and the second image. Through image visual processing, seve...
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The invention relates to a coordinate and displacement error detection and compensation method for a microscope operating system. According to the method, the image identifying and stitching operation is conducted through the image visual processing process. After that, the deflection angle theta between a screen coordinate system and a platform coordinate system, and the actual movement displacement of a platform can be accurately calculated. The difference between the actual movement displacement of the platform and the theoretical displacement is just the displacement error. Meanwhile, based on derived formula compensation coordinates and the displacement error, only the platform needs to be moved to a corresponding compensation position during the microscope operating process. In this way, a cell (operation object) can be accurately positioned at any given position of a screen. The above method is simple in operation and high in positioning precision. In addition, mechanical equipment is not damaged at all, and the precision of the equipment is not influenced. Moreover, the method has a certain flexible, high in universality, and wide in applicable range.
Description
technical field [0001] The invention relates to an automatic micromanipulation technology, in particular to a method for detecting and supplementing coordinate and displacement errors in a micromanipulation system based on computer image visual feedback. Background technique [0002] With the rapid development of the modern biomedical field, micromanipulation technology has been widely used. Micromanipulation technique refers to a method of operating cells or early embryos under a microscope using a micromanipulator. way. Including nuclear transfer, microinjection, microdissection, micropositioning, chimera technology, embryo transfer, etc. Since the object of micromanipulation is invisible to the naked eye, the precision of micromanipulation plays an important role in the operation. In the process of micromanipulation, the working platform of the equipment receives commands from one direction (X or Y) or two directions (XY) and moves. We define the coordinate system where...
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IPC IPC(8): G01B11/26G01B11/02
CPCG01B11/022G01B11/26
Inventor 胡绍燕翟荣安陈瑞华汝长海
Owner SOOCHOW UNIV AFFILIATED CHILDRENS HOSPITAL