Marasmiellus androsaceus strain and application thereof

A technology of Anluo Xiaopi and strains, applied in the field of microorganisms, can solve the problems of low yield, complicated separation process, and small added value, and achieve high Anluo Xiaopi polysaccharide yield and simple operation

Inactive Publication Date: 2016-04-06
西藏天虹科技股份有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The Chinese invention patent application No. 201410337924.0 provides a method of liquid fermentation, but the method is fermented with common strains, and the yield is low, and the enzymatic method is used in the extraction process of Anluo parsley polysaccharides to separate The process is complex and the content is low
At present, the main domestic manufacturer is Shanghai Kangzhou Polysaccharide Co., Ltd. The polysaccharide content of Anluo Xiaopi umbrella is 20-40%, the output is low, and the added value is small

Method used

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  • Marasmiellus androsaceus strain and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 Utilizes the mutagenesis of the starting strain to obtain the mutant strain H123

[0030] The starting strain of Anluo parsley was obtained by tissue separation method, inoculated on the wort fixed slant medium, cultured at 26°C for 48 hours, and then stored in the refrigerator at 4°C for later use. Take 8mL of spore suspension in a 6cm plate, stir it magnetically, and irradiate it with a UV lamp at 30cm for 20s, then perform the first round of irradiation. The first mutant strain was subjected to the second round of ultraviolet radiation mutagenesis for 30 seconds, and the high-yielding strain H125, which grew well and produced high levels of polysaccharides from the umbrella parsley, was obtained through primary and secondary screening.

[0031] The components in the slant medium are calculated by weight percentage: 3% glucose, 1% yeast extract, 1% beef extract, 0.1% sodium chloride, 0.05% potassium dihydrogen phosphate, 1.0% agar powder, and the pH value...

Embodiment 2

[0034] Change the composition of the slant medium, and cultivate the high-yield strain and the starting strain respectively. Each component in the slant culture medium is designed by weight percentage: glucose 2%, yeast extract 2%, beef extract 1%, sodium chloride 0.0%, potassium dihydrogen phosphate 0.05%, agar powder 1.0%, pH value 5.0 to 6.0. The sugar production of the high-yielding strain was increased by 18% compared with the starting strain.

Embodiment 3

[0036] figure 1 Provided is a process flow for producing the polysaccharide of Anluo puffyman in one embodiment.

[0037] Step 1. Cultivate high-yield strain H125

[0038] Step ① Pick from the mother seed test tube, and culture the fresh high-yielding strain H125 in a primary shake flask. In the primary shake flask culture process, 50mL seed culture medium was packed in a 500mL Erlenmeyer shake flask, 23°C, 150rpm shaking culture for 48h, and the primary shake flask strain was obtained.

[0039] In step ②, carry out secondary shake flask culture on the strains of the primary shake flask. Put 200mL of seed culture medium in a 1000mL Erlenmeyer shake flask, insert 50mL of the strain of the first shake flask, and culture at 24°C and 150rpm for 36h with shaking to obtain the strain of the second shake flask.

[0040] Step ③ Carry out primary seed culture on the secondary shake flask strain. The secondary shake flask strains were inserted into the 100L fully automatic fermenter...

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Abstract

The invention discloses a marasmiellus androsaceus strain. The preservation number of the marasmiellus androsaceus strain is CGMCC No.11734. The invention further discloses a method for applying the marasmiellus androsaceus strain in producing marasmiellus androsaceus polysaccharide. The process of hot water extraction, ultrafitration separation and ethanol precipitation is adopted, the extraction method is easy to implement, the content of marasmiellus androsaceus polysaccharide in a separated product is not lower than 60%, and the polysaccharide extraction rate is not lower than 12.3%.

Description

technical field [0001] The present invention relates to the field of microbes, in particular to a Pymella anluo strain and its application. Background technique [0002] Anluo Xiaopijiao polysaccharide is one of the important active substances of Anluo Xiaopijiao, which has various activities such as improving immunity, anti-tumor, and anti-aging. At present, at home and abroad, most of the polysaccharides are extracted from the fruiting bodies of the fungus Anluo. There are mainly chemical methods, enzymatic methods and physical methods for the extraction of polysaccharides from Anluo Xiaopijiao. Among them, the enzymatic method has strong specificity, but it is difficult to determine a suitable degrading enzyme for an unknown polysaccharide, and the cost is high and the conditions are harsh. [0003] Li Xuemei et al. studied the relationship between reducing sugar and mycelium dry weight, pH value and yield in the liquid fermentation process of Dermatomida anluo, which p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C08B37/00C12R1/645
CPCC08B37/0003C12N1/145C12R2001/645
Inventor 张春颖
Owner 西藏天虹科技股份有限责任公司
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