Method for high throughput screening of saccharomyces cerevisiae
A beer yeast and purpose technology, applied in the field of high-throughput screening of beer yeast, can solve the problems of slow screening work, heavy workload, and limited number of screenings, and achieve the effects of reducing screening work intensity, improving efficiency, and increasing the number of colonies
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[0033] The preparation method of the wort medium in the following examples: the wort (Yanjing Brewery Co., Ltd.) was sterilized and obtained.
[0034] The preparation method of the wort agar medium in the following examples: add 2% agar powder to the liquid wort medium.
[0035] The deposit number of brewer's yeast strain 8902 in the following examples is CGMCC No.1784.
[0036] The preservation number of brewer's yeast strain 0002 in the following examples is CGMCC No.10572.
[0037] The brewer's yeast strain 68obg in the following examples is a product of the Berlin Institute of Brewing, Germany, product number: 016719.
[0038] The brewer's yeast strain O.K.3 in the following examples is a product of the Berlin Institute of Brewing, Germany, product number: 016720.
Embodiment 1
[0039] Embodiment 1, the optimization of brewer's yeast culture parameter
[0040] 1. Parameter optimization
[0041] 1. Treatment of strains
[0042] The brewer's yeast strain (8902CGMCCNo.1784) to be screened is activated and expanded to obtain a cultured bacterial suspension after activation. The specific steps are as follows:
[0043] (1) Use an inoculation loop to pick up a loop of the strains stored on the slant and add it to a test tube containing 10mL of 12°P wort medium, and incubate at 25±1°C for 24 hours.
[0044] (2) Gently oscillate the culture solution in the test tube after the cultivation, and add 1 mL of it to a test tube containing 10 mL of 12°P wort medium with a sterile pipette, and incubate at 25±1°C for 24 hours after oscillating evenly.
[0045] (3) Gently oscillate the test tube culture solution after the end of the culture, and then put them all into a small Erlenmeyer flask filled with 100mL of 12°P wort medium, and incubate at 25±1°C for 24 hours a...
Embodiment 3
[0064] Embodiment 3, the application of the method for high-throughput screening brewer's yeast in screening brewer's yeast
[0065] 1, Erlenmeyer flask fermentation method (contrast)
[0066] In this example, the traditional shaking flask screening (triangular flask fermentation method) was used as a comparison, and the brewer's yeast strains (8902, 68obg, 0002 and O.K.3) were fermented and cultured, and the brewer's yeast was screened according to the diacetyl content or OD value in the fermentation broth strain. Specific steps are as follows:
[0067] (1) The brewer's yeast strains (8902, 68obg, 0002 and O.K.3) were activated and expanded (see step 1 in Example 1 for the expansion method), and the activated bacterial suspensions were respectively obtained.
[0068] (2) Fermentation culture
[0069] Inoculate the bacterial suspension obtained in the above step (1) into the Erlenmeyer flasks equipped with 350mL wort medium (12°P) for fermentation and culture respectively, ...
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