A kind of Japanese medical leech spawning method and its special substrate
A Japanese medical leech and substrate technology, which is applied in animal husbandry and other directions, can solve the problems of poor reuse rate, no egg cocoon formation, restricting Japanese medical leech, etc. Effect
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Embodiment 1
[0011] Example 1 Japanese medical leech spawning condition investigation 1
[0012] 1 material
[0013] 1.1 Test materials: Japanese medical leeches (provided by Chongqing Zhi Microbial Technology Co., Ltd.), coconut peat, and crushed forage.
[0014] 1.2 Test equipment: oxygenation pump, spawning water tank (1.0m (length) × 0.7m (width) × 0.7m (height))
[0015] 2 methods
[0016] 2.1 Selection of leeches and experimental design
[0017] From the Japanese leech breeding base, select Japanese leeches over 1 year old, with an individual weight of more than 0.5 grams, strong physique, sensitive response, and obvious reproductive rings for the oviposition test. After the experiment of each group, count the eggs under different conditions Condition.
[0018] The spawning water tank that is used for experiment, half puts water (the water level height is at 20cm), and the other half is covered with substrate. The part covered with the substrate is specifically: laying pebbles a...
Embodiment 2
[0041] Example 2 Japanese medical leech spawning condition investigation 2
[0042] According to the single factor investigation conditions described in Example 1, under conditions such as different spawning pH value conditions, spawning environment temperature, proportioning of spawning substrates, and water content of spawning substrates, the spawning rate of each group was counted, and the design Orthogonal experiments are shown in Table 4 and 5.
[0043] Table 4 Orthogonal design experiment factor levels
[0044]
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