Anti-glioma drugs based on Venenum Bufonis extract and preparation method thereof
A brain glioma, extract technology, applied in the field of anti-tumor drugs, can solve problems such as unsatisfactory curative effect and poor prognosis
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experiment example 1
[0050] The preparation of bufolin, cinobufagin and bufabugenin combined content greater than 95% of the toad venom extract comprises the following steps:
[0051] (1) Pulverize the toad venom medicinal material into coarse powder, pass through a 60 mesh sieve, and dissolve in ethanol with a volume concentration of 80%. Wherein, the quality of ethanol is 10 times that of the coarse powder, and is dissolved by heating in a water bath, and the heating temperature of the water bath is 80 ℃, the heating time is 1h; then suction filtration, the ethanol in the filtrate is evaporated and dried to obtain the crude extract.
[0052] (2) Add the crude extract to distilled water, wherein the quality of distilled water is 10 times of the quality of the crude extract, and ultrasonically suspend to obtain a suspension; then add ethyl acetate for extraction, wherein the quality of ethyl acetate is 10 times that of the quality of the suspension times, the extract was obtained; the ethyl acetat...
experiment example 2
[0059] The common solution (bufadienolidessolution, BU-S) of the above-mentioned toad venom extract is used for anti-glioma drugs, and its 5mL standard dosage composition is: 2.5mg bufadienolide extract, 2.5mL the first solvent propylene glycol, and the rest is the second Solvent injection water. Wherein, the comprehensive content of bufolin, cinobufagin and lipobufagenin in the toad venom extract is greater than 95%. This experimental example 2 uses the toad venom extract prepared in experimental example 1.
[0060] The preparation method of the common solution of the above-mentioned toad venom extract comprises the following steps: weigh 2.5 mg of the toad venom extract, put it in a 5 mL measuring bottle, add 2.5 mL of propylene glycol, dissolve it by ultrasonic, add water for injection to dilute to a constant volume of 5 mL, shake well, and use 0.22 Filter through a μm filter membrane.
experiment example 3
[0062] A kind of anti-glioma drug based on toad venom extract of the present invention is bufaxin submicroemulsion preparation (BU-SCP), and its 100mL standard dose components are: 50mg toad venom extract, 10g medium chain triglyceride Ester (MCT), 3g lecithin, 0.2g poloxamer 188 (F-68), 2.5g glycerin, 0.05g sodium oleate, and the rest is water for injection. Wherein, the comprehensive content of bufolin, cinobufagin and lipobufagenin in the toad venom extract is greater than 95%. This experimental example 3 uses the toad venom extract prepared in experimental example 1.
[0063] The preparation method of the above-mentioned bufaxin submicroemulsion preparation (BU-SCP) is characterized in that the preparation of its 100mL standard dose comprises the following steps:
[0064] (1) Add 3g of lecithin and 50mg of toad venom extract to 10g of medium-chain triglyceride (MCT) respectively, heat and stir to dissolve, and obtain a clear drug-containing oil phase; wherein, the heating...
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