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Application of tanshinone compounds to preparation of antitumor medicines

A technology of anti-tumor drugs and compounds, applied in the application field of tanshinone compounds in the preparation of anti-tumor drugs

Active Publication Date: 2017-06-27
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the research objects of antitumor activity of tanshinone compounds mainly focus on active molecules with high content and wide application, and there are few reports on the antitumor activity of trace tanshinone compounds

Method used

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  • Application of tanshinone compounds to preparation of antitumor medicines
  • Application of tanshinone compounds to preparation of antitumor medicines
  • Application of tanshinone compounds to preparation of antitumor medicines

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0032] The inhibitory effect of CCK-8 method Tanshinone compounds on tumor cell proliferation

[0033] The four tanshinone compounds prepared in Example 1.1 were prepared into different concentrations of drug-containing solutions with serum-free medium. Human lung cancer cell A549 and pancreatic cancer cell DT6606 were used as experimental subjects. The logarithmic growth phase was in good condition. The A549 cells and DT6606 cells were seeded in a 96-well plate with a seeding concentration of 5000 cells / well and placed in a conventional cell incubator for 12 hours. The tumor cells were processed according to the previous dosing schedule, and the drug-free medium was used as a negative control. After 24 hours of culturing, the CCK-8 method was used to determine the cell viability after drug action, and the in vitro anti-tumor activity of the four tanshinone compounds was analyzed. The specific steps are:

[0034] a) Administration: As the four tanshinone compounds choose to use 1...

experiment example 2

[0045] Annexin V / PI double staining method to evaluate the apoptosis-inducing effect of four tanshinone compounds on lung cancer cell line A549

[0046] Take A549 cells in logarithmic growth phase and adjust the cell density to 2×10 4 Pieces / well, inoculated in a 24-well plate, 1 mL per well, after normal incubation for 12 hours, discard the medium, and add different concentrations (1 μM, 5 μM and 10 μM) of the four tanshinone compounds prepared in Example 1.1 for 24 hours. And set up a normal control group, with three replicate holes in each group.

[0047] Discard the supernatant in the culture plate, wash the cells twice with PBS, add trypsin without EDTA for 1 min, stop with serum-containing medium, collect the cells in the same centrifuge tube, centrifuge at 1000 rpm for 3 min, discard the supernatant .

[0048] After adding PBS, centrifuge at 1000 rpm for 3 min, and discard the supernatant. Wash twice, add the pre-prepared 1×AnnexinV Binding Solution to make the final concent...

Embodiment 1

[0054] Method for preparing and chemical structure identification of said tanshinone compounds

[0055] 1.1 Extraction and separation

[0056] The crude extract of the ethyl acetate part of Salvia miltiorrhiza was 2.5 kg, and enough dichloromethane was added to dissolve it completely, and then transferred to a separatory funnel. Add 2 times the volume of water to the separatory funnel and mix gently, stand still and separate to obtain the dichloromethane layer, which is transferred to a round bottom flask and concentrated to the state of extract using a rotary evaporator to obtain ethyl salvia miltiorrhiza. Site extract.

[0057] Dissolve the extract of the ethyl acetate part of Salvia miltiorrhiza with an appropriate amount of ethyl acetate, mix it with 4.9 kg of sample-mixing silica gel (200-300 mesh) and grind it. After the solvent has evaporated, pass through a 60-mesh sieve and load the sample. Use different ratios of petroleum ether: ethyl acetate (20:1, 10:1, 5:1, 1:1) to pe...

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Abstract

The invention relates to the field of natural medicines, and provides application of tanshinone compounds to preparation of antitumor medicines. Tumors include lung cancer, prostate cancer, breast cancer, colon cancer, cervical cancer, liver cancer, pancreatic cancer, stomach cancer and the like. Experimental results show that the tanshinone compounds are concentration-dependent and time-dependent, can inhibit growth of tumor cells, and can significantly induce apoptosis of the tumor cells.

Description

Technical field [0001] The invention relates to the field of natural medicines and anti-tumor compounds, in particular to the application of tanshinone compounds in the preparation of anti-tumor drugs. Background technique [0002] Malignant tumors are the main cause of global morbidity and mortality. Relevant statistics show that in 2012, there were approximately 3.586 million new cases of malignant tumors in my country and 2.187 million deaths. It is estimated that the number of new cases will increase by about 70% in the next 20 years [Analysis of the incidence and death of malignant tumors in China in 2012. Chinese Cancer, 2016, 25(1):1-8]. At present, the main treatment methods for malignant tumors are surgical resection and chemotherapy, among which surgical resection is the first choice. However, malignant tumors are prone to remote metastasis and local infiltration in the early stage, which brings great difficulties to surgical resection. The current first-line chemother...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61K31/122A61P35/00
CPCA61K31/122A61K31/343
Inventor 陈君张效威李雪岩杨琳
Owner CHINA PHARM UNIV
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