Droplet generation device and method used for digital PCR

A droplet generation and generation device technology, applied to laboratory containers, chemical instruments and methods, laboratory utensils, etc., to achieve the effects of small variance, improved real-time performance, and good repeatability of results

Inactive Publication Date: 2016-04-13
SUZHOU BEIHE MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this technology has not yet appeared in the market

Method used

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Embodiment Construction

[0010] The present invention will be described in more detail below in conjunction with preferred embodiments.

[0011] Droplet generation consists of two steps: raw droplet generation and droplet splitting. Droplet splitting is a technology that splits one droplet into two under the action of mechanical force by changing the channel design. Droplet splitting can be designed into a multi-level structure, thereby achieving an exponential increase in the number of generated droplets. That is, the original droplet generates two primary sub-droplets through the primary structure; each primary sub-droplet generates two secondary sub-droplets through the secondary structure; each secondary sub-droplet can generate through the tertiary structure Two tertiary sub-droplets. In this way, the number of droplets generated increases exponentially with the increase in the structural complexity of the microfluidic system, while the volume of the droplets decreases rapidly. The smaller the...

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Abstract

The invention relates to a droplet generation device and a droplet generation method used for digital PCR. The invention discloses the droplet generation method used for digital PCR. According to the invention, a droplet generation process comprises the two steps: original droplet generation, and droplet break up. In the droplet break up step, one droplet is broken up into two under mechanical force. With the method, a large amount of droplets can be generated fast and stably. The volumes of the droplets are uniform, such that a good result repeatability is ensured, and variance is small. Droplet generation time consumption is reduced, needed experiment time is saved, and timeliness is improved.

Description

technical field [0001] The invention relates to a droplet generating device and method for digital PCR. Background technique [0002] Stable and rapid generation of a large number of micro-droplets is very important for droplet digital PCR. The volume of the droplet is uniform, ensuring good repeatability and small variance of the results. The number of droplets determines the range of template DNA quantities that can be measured in the linear range. The droplet generation is fast, and the time-consuming droplet generation is shortened, which can save the time required for the experiment and improve real-time performance. However, this technology has not yet appeared in the market. Contents of the invention [0003] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a method capable of stably and rapidly generating a large number of micro-droplets. [0004] To achieve the above object, the present invention adopts the follow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
Inventor 蒋跃明何云刚
Owner SUZHOU BEIHE MEDICAL TECH CO LTD
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