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Method for detecting angiogenesis promoting capacity of MSC through VCAM-1

A technology of VCAM-1 and angiogenesis, applied in the field of stem cells, can solve the problems of lack of detection and evaluation of the ability to promote angiogenesis, and hinder the clinical application of cells

Active Publication Date: 2016-04-13
北京汉氏干细胞科技有限公司
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  • Abstract
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Problems solved by technology

However, there is currently a lack of methods to detect and evaluate the pro-angiogenic ability of MSCs, which hinders the clinical application of cells.

Method used

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  • Method for detecting angiogenesis promoting capacity of MSC through VCAM-1
  • Method for detecting angiogenesis promoting capacity of MSC through VCAM-1
  • Method for detecting angiogenesis promoting capacity of MSC through VCAM-1

Examples

Experimental program
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Effect test

Embodiment

[0036] Step 1. Detect the ability of sample cells to promote endothelial cell proliferation

[0037] Umbilical vein endothelial (HUVEC) cells were planted in the lower cavity of the transwell system, and MSC cells were planted in the upper cavity. Three replicate wells were set for each group, and the proliferation of HUVEC was detected after co-culture. Because endothelial cells are cells that directly form blood vessels, the effect of sample cells (MSCs) on endothelial cells reflects its role in promoting angiogenesis. In the experiment, 8 MSC cells from different individuals were selected for the experiment. The cells used for detection were all P5 generation. The experimental results showed that different cells promoted the proliferation of HUVEC to varying degrees, but there were individual differences ( figure 1 ), see Table 1 for specific data.

[0038] Table 1. Promoting HUVEC proliferation rate of different sample cells

[0039]

[0040] Step 2, 3, 4.ELISA detect...

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Abstract

The invention discloses a method for detecting the angiogenesis promoting capacity of an MSC through VCAM-1. The method is characterized in that a new purpose of known protein VCAM-1 is found, the protein expression of VCAM-1 is detected through an ELISA, the total protein content is measured through a Bradford method, and the angiogenesis promoting capacity of the cell is reflected through the protein content of VCAM-1 in every 1 g of total protein. The angiogenesis promoting capacity of the MSC can be quantified through the method.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a method for detecting the ability of MSCs (mesenchymal stem cells) to promote angiogenesis by using VCAM-1 (vascular cell adhesion molecule-1). Background technique [0002] Vascular endothelial cell adhesion molecule-1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. There are two isoforms of VCAM-1 with six or seven extracellular Ig-like domains produced by alternative splicing. Subtype. VCAM-1 is an endothelial cell ligand that binds to the VLA-4 antigen and α4β7 integrin expressed on leukocytes, thereby mediating leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 is induced on endothelial cells in response to inflammatory bowel disease, atherosclerosis, allograft rejection, infection and asthma. In these responses, VCAM-1 constitutes a scaffold for leukocyte migration. VCAM-1 also activates signals ...

Claims

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Application Information

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IPC IPC(8): C12Q1/06G01N33/68
CPCG01N33/5064G01N33/5073G01N33/68
Inventor 梁璐赵钦军韩之海
Owner 北京汉氏干细胞科技有限公司
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