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Application of rice cell cycle protein OsCYCP4;1 and method for improving deficient phosphorus stress resistance of rice

A cell cycle and low phosphorus tolerance technology, applied in the field of plant genetic engineering, can solve problems such as no CYCP family connection found, and achieve the effect of improving low phosphorus tolerance and high tolerance

Active Publication Date: 2016-04-20
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
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Problems solved by technology

But so far no link between the CYCP family in Arabidopsis and the response to phosphorus starvation signals has been found

Method used

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  • Application of rice cell cycle protein OsCYCP4;1 and method for improving deficient phosphorus stress resistance of rice
  • Application of rice cell cycle protein OsCYCP4;1 and method for improving deficient phosphorus stress resistance of rice
  • Application of rice cell cycle protein OsCYCP4;1 and method for improving deficient phosphorus stress resistance of rice

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Embodiment

[0026]A method for improving the resistance of rice to low phosphorus stress, by knocking out the rice cell cycle gene OsCYCP4; 1 encoding gene of rice cell cycle protein OsCYCP4; The ability of low phosphorus stress is improved.

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Abstract

The invention discloses application of a rice cell cycle protein OsCYCP4;1 and a method for improving deficient phosphorus stress resistance of rice. Expression of OsCYCP4;1 is induced by deficient phosphorus stress on both the transcription level and the translation level. An over-expressed OsCYCP4;1 gene restrains rice growth, and it is proved that rice growth is negatively adjusted by the over-expressed OsCYCP4;1 gene. Under the normal cultivation conditions, compared with wild type mutants, OsCYCP4;1 afunction mutants have no remarkable phenotypic difference but have remarkably higher phosphorus content. Under the phosphorus deficiency conditions, OsCYCP4;1 afunction mutants reduce the sensitivity of low-phosphorus inhibit aboveground growth. Guarantees are provided for improving the low phosphorus tolerance of plants and cultivating rice new species suitable for phosphorus deficient soil.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to the application of a rice cell cycle protein OsCYCP4; 1 and a method for improving rice tolerance to low phosphorus stress. Background technique [0002] Phosphorus is one of the macroelements necessary for all life. Phosphorus not only constitutes important macromolecules in cells including DNA, RNA, ATP and phospholipids, but also participates in regulating important physiological and biochemical processes such as signal transduction, energy metabolism and photosynthesis (AbelS (2011) Phosphatessensinginrootdevelopment.CurrOpinPlantBiol14 :303-309). With the continuous improvement of the research level of molecular biology, we have a certain understanding of the complex physiological and biochemical processes of phosphorus uptake and metabolism in plants. Genes involved in response to low phosphorus stress in plants are continuously being discovered, includi...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8205C12N15/8271C12N2800/80C12N2810/10
Inventor 易可可邓敏娟王芳
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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