Determination of multi-index components, fingerprint construction and preparation method of Yigan Mingmu Oral Liquid

A kind of Mingmu oral liquid, multi-index technology, applied in the field of traditional Chinese medicine

Active Publication Date: 2017-11-14
BEIJING HEBABIZ BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since then, there has been no disclosure or revelation of in-depth research on Yigan Mingmu Oral Liquid

Method used

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  • Determination of multi-index components, fingerprint construction and preparation method of Yigan Mingmu Oral Liquid
  • Determination of multi-index components, fingerprint construction and preparation method of Yigan Mingmu Oral Liquid
  • Determination of multi-index components, fingerprint construction and preparation method of Yigan Mingmu Oral Liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] The processing of embodiment 1 sample

[0077] Yigan Mingmu oral liquid sample (for determination of index components): Accurately measure 1 mL of Yi Gan Ming Mu oral liquid (available from Guangxi Huibaoyuan Pharmaceutical Co., Ltd.), put it in a 10 mL volumetric flask, add methanol to dilute to 10 mL , shake well, weigh, sonicate for 10min (power 100W, frequency 40kHz, temperature 20 ℃), after standing to room temperature, weigh, make up the lost weight with methanol, and obtain the test solution (all test samples were prepared detected within 24 hours).

[0078] Sample of Yigan Mingmu Oral Liquid (for fingerprint determination): Take 5 mL of Yigan Mingmu Oral Liquid, add 2 times the volume ratio of water-saturated n-butanol for extraction, extract 3 times, combine the extracts, and recover the solvent under reduced pressure , redissolved in methanol, transferred to a 10mL measuring bottle, constant volume with methanol to the mark, and filtered through a microporous...

Embodiment 2

[0080] The determination of embodiment 2 detection conditions

[0081] 1. Determination of detection wavelength

[0082] 1. Measurement wavelength of each index component

[0083] High-performance liquid chromatography detects each index component: Chromatographic column: Inertsil ODS-SP chromatographic column (250mm * 46mm, 5 μ m); Mobile phase A acetonitrile, mobile phase B 0.05% (v / v) phosphoric acid aqueous solution, gradient elution, The elution gradient is shown in Table 3; the flow rate is 0.8mL min -1 ; Column temperature 35°C.

[0084] table 3

[0085] time (minutes)

[0086] Experimental results

[0087] The reference substance solution was detected by a diode array detector, and it was found that chlorogenic acid, ferulic acid and nobiletin had strong absorption at 325nm, luteolin had strong absorption at 348nm, and hesperidin had strong absorption at 284nm. Strong absorption, paeoniflorin has a strong absorption at 230nm. The diode array detector ca...

Embodiment 3

[0115] Example 3 Research on the content determination of each index component of Yigan Mingmu Oral Liquid

[0116] On the basis of the determination conditions of high performance liquid chromatography (HPLC) determined in Example 2, the determination of each index component of Yigan Mingmu Oral Liquid was studied in depth.

[0117] 1. Standard curve and linear range

[0118] Dilute the samples of each index component according to the concentrations shown in Table 7-1~7-6, and perform HPLC detection. The data results are shown in Table 7-1~7-6, and the standard curve is drawn in Table 8.

[0119] Table 7-1 Chlorogenic acid standard curve data

[0120] C (μg / mL)

2.22

4.44

6.66

8.88

11.1

2.22

A

122798

395149

663898

938840

1212218

122798

[0121] Table 7-2 Paeoniflorin standard curve data

[0122]

[0123] Table 7-3 Ferulic acid standard curve data

[0124]

[0125] Table 7-4 luteolin standard curve data

[0126]...

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Abstract

The invention provides a method for determining multi-index components or fingerprints of the Yigan Mingmu Oral Liquid, which targets chlorogenic acid, luteolin, ferulic acid, nobiletin, hesperidin and paeoniflorin. The determination method has the advantages of simple and convenient operation, strong specificity, good stability, high precision, good reproducibility, and high sample recovery rate. In addition, the present invention also provides a method for preparing the Yigan Mingmu oral liquid including the steps of the determination method, and the prepared Yigan Mingmu oral liquid and the like.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to a method for simultaneously measuring multiple index components of Yigan Mingmu Oral Liquid and a preparation method of Yigan Mingmu Oral Liquid. Background technique [0002] Yigan Mingmu Oral Liquid (see National Medical Products Administration Standard (Trial) ws-5054(B-0054)-2002) has been approved for marketing, and its prescription includes rehmannia glutinosa, angelica, medlar, white peony root, etc. It is a herbal medicine with a strong taste and has the effect of nourishing the liver and kidney. It is suitable for symptoms such as weakness of the waist and knees, dizziness, memory loss, and fatigue. [0003] Due to the wide variety of crude drugs, the active ingredients of each crude drug are difficult to count, and the process stability between each production batch is not strong, resulting in a large difference in the active ingredients of each c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02A61K36/8968A61K9/08A61P25/00A61P27/10A61P27/02
Inventor 周骅谢百波臧新钰程林友梁光清
Owner BEIJING HEBABIZ BIOTECHNOLOGY CO LTD
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