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Preparation method of traditional Chinese medicine and pollen feed for bumblebees

A technology of pollen and traditional Chinese medicine, applied in the biological field, can solve the problems of many bumblebees, high labor intensity, weak disease resistance of worker bees, etc., and achieve the effects of simple process operation, low feed cost and long survival period.

Inactive Publication Date: 2016-04-27
郭礼强
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The development of the economy and the improvement of people's living standards have promoted the expansion of the greenhouse planting area year by year. Because indoor planting crops are difficult to accept natural pollination, traditional agriculture often uses artificial, mechanical vibration and hormone pollination, which is labor-intensive, costly and deformed. , affecting the yield and quality of fruit, and the use of bumblebees to pollinate indoor crops has developed into a necessary supporting measure for modern agriculture
Due to the late start of bumblebee breeding technology in our country, there is no mature and effective bumblebee breeding technology at present. Only a few countries in the world, such as the United Kingdom and the Netherlands, have mastered bumblebee artificial breeding technology. Therefore, importing high-quality bumblebees from abroad has become an urgent need for some vegetable and fruit growing enterprises. However, the pesticide residues in the pollen on the market, and if the imported bumblebees are not fed well in the feed formula, it is easy to cause the disadvantages of short hive survival period and weak disease resistance of worker bees. There are many bumblebees (no pollination), which raises the The cost of pollination hinders its rapid adoption

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, a kind of preparation method of Chinese medicine pollen feed for bumblebees, comprises the following steps:

[0036] a. Strains: Saccharomyces (edible Angel Yeast); Pediococcus pentosaceae (strain preservation number CGMCC No. 10573), stored in a 4°C inclined plane refrigerator.

[0037] b. Yeast medium (seed medium): Peel and cut potatoes into pieces, add 500mL water for every 200g, boil for 30min. Filter through double-layered gauze into a beaker, add 20g sucrose, 5g rape pollen, add water to 1L, put each 100mL solution into a 250mL conical flask, and autoclave at 121°C for 20min.

[0038] c. Pediococcus pentosaceae medium (seed medium): Take 5.0g of soybean peptone, 5.0g of beef extract powder, 5.0g of yeast extract, 20g of glucose, and 20g of lactose, add water to 1L, and divide each 100mL solution into 250mL triangles Bottles, autoclaved at 121°C for 20 minutes.

[0039] d. Basic culture medium: take 35g of glucose, 35g of fructose, 10g of sucrose, ...

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Abstract

The invention discloses a preparation method of traditional Chinese medicine and pollen feed for bumblebees. The method comprises steps as follows: strains are selected; a yeast culture medium is prepared; a Pediococcus pentosaceus culture medium is prepared; a basic culture medium is prepared; a fermentation culture medium is prepared; seeds are cultured; the fermentation culture medium is inoculated with 20 mL of cultured yeast strains and placed in a fungus incubator at the temperature of 28 DEG C for culture, the cultured fermentation culture medium is heated to the temperature of 50 DEG C, the temperature is kept for 3 h and then decreased to 32 DEG C, and then 40 mL of Pediococcus pentosaceus strains are added, cultured at the temperature of 32 DEG C for 1 d and then continuously heated to the temperature of 35 DEG C for anaerobic culture. The cost is reduced by 1 / 4, 56% of worker bees have the survival time of 10-12 weeks, the bumblebees have high activity and disease resistance, and the reproductive capacity of the bumblebees is 51.9% higher than that of bumblebees only collecting strawberry pollen and 18.5% higher than that of bumblebees which collect pollen independently and are directly fed with pollen in a supplementary manner in honeycombs.

Description

field of invention [0001] The present invention relates to a kind of preparation method for producing bumble bee feed, specifically, relate to a kind of bumble bee ( Bombster resris ) The preparation method of pollen feed with traditional Chinese medicine belongs to the field of biotechnology. Background technique [0002] Bumblebee is a kind of polyphagous social insect. It has the characteristics and advantages of strong collection ability, light resistance, poor phototaxis, and strong adaptability to low temperature. It can still pollinate under the harsh conditions where bees do not pollinate, because bumblebees are not as sensitive as bees The information exchange system can concentrate on collecting pollination on crops at room temperature, thus becoming a more ideal pollinator than honeybees in greenhouses. The development of the economy and the improvement of people's living standards have promoted the expansion of the greenhouse planting area year by year. Because ...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/18A23K50/90A23K40/00
CPCA61K36/064A23V2400/427
Inventor 郭礼强田国宁郝莹赵晗崔晓娜张丽萍王梅玲
Owner 郭礼强
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