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Application of rice cell cycle protein oscycp4; 4 and the method of improving rice tolerance to low phosphorus stress

A technology of cell cycle and low phosphorus tolerance, applied in the field of plant genetic engineering, can solve the problem of no CYCP family connection found, and achieve the effect of improving low phosphorus tolerance and high tolerance

Active Publication Date: 2018-11-27
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far no link between the CYCP family in Arabidopsis and the response to phosphorus starvation signals has been found

Method used

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  • Application of rice cell cycle protein oscycp4; 4 and the method of improving rice tolerance to low phosphorus stress
  • Application of rice cell cycle protein oscycp4; 4 and the method of improving rice tolerance to low phosphorus stress
  • Application of rice cell cycle protein oscycp4; 4 and the method of improving rice tolerance to low phosphorus stress

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Embodiment

[0026] A method for improving rice tolerance to low phosphorus stress, by knocking out the rice cell cycle gene OsCYCP4;4 encoding the rice cell cycle protein OsCYCP4; The ability of low phosphorus stress was improved.

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Abstract

The present invention discloses rice cyclin OsCYCP4 ; 4 application and a method for improving rice tolerance to low phosphate stress, and OsCYCP4 ; 4 transcription level expression and OsCYCP4 ; 4 translation level expression are both induced by phosphorus starvation stress. Inhibition of overexpression of OsCYCP4; 4 gene on the growth of rice proves that the overexpression of OsCYCP4; 4 gene negatively regulates the growth of rice. Under normal culture conditions, and compared with a wild type, an OsCYCP4; 4 functional deletion mutant has no obvious phenotypic differences, but the phosphorus content of the mutant is significantly higher than that of the wild type. Under phosphorus deficiency conditions, the OsCYCP4; 4 functional deletion mutant reduces sensitivity of low-phosphorus inhibition on growth of overground parts. A guarantee for improvement of plant tolerance to low phosphorus and cultivation of new rice varieties suitable for phosphorus poor soils is provided.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to the application of a rice cell cycle protein OsCYCP4;4 and a method for improving rice tolerance to low phosphorus stress. Background technique [0002] Phosphorus is one of the large elements necessary for all life. Phosphorus not only constitutes important macromolecules including DNA, RNA, ATP and phospholipids in cells, but also participates in the regulation of important physiological and biochemical processes such as signal transduction, energy metabolism and photosynthesis (Abel S (2011) Phosphate sensing in root development. Curr OpinPlant Biol 14:303-309). With the continuous improvement of the research level of molecular biology, we have a certain understanding of the complex physiological and biochemical processes of phosphorus absorption and metabolism in plants. Genes involved in the response to low phosphorus stress in plants are constantly being...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8271
Inventor 易可可邓敏娟王芳黄继荣刘洪家
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES