Animal lymphocyte separation liquid and preparation method thereof

Abstract

The invention discloses animal lymphocyte separation liquid, which consists of the following components in percentage by mass: 10% of sodium diatrizoate, 5.2-4.4% of ficoll, 0.8-1.6% of a hydrophilic high-molecular polymer of non-glycosyl units and the balance of de-ionized water free from a heat source, totaling 100%, wherein the total concentration of the ficoll and the hydrophilic high-molecular polymer of the non-glycosyl units is 6%; and the specific weight of the animal lymphocyte separation liquid is 1.076-1.078g / ml at 20 DEG C. The animal lymphocyte separation liquid, which is low in cost and high in efficiency, is prepared by replacing expensive meglumine diatrizoate with sodium diatrizoate and by mixing and combining the sodium diatrizoate with the ficoll, and on the basis of a consistent separation effect of the sodium diatrizoate with the meglumine diatrizoate, the production cost is significantly reduced; and by adding the hydrophilic high-molecular polymer of the non-glycosyl units, such as polyvinylpyrrolidone, polyvinyl alcohol and polypropylene glycol, the cell banding time of separating lymphocytes with the low-cost prescription is significantly shortened, meanwhile the quality of the obtained lymphocytes is significantly improved, platelet contamination is nearly avoided.

Description

technical field [0001] The invention relates to an animal lymphocyte separation liquid and a preparation method thereof, belonging to the technical field of biomedicine. Background technique [0002] The traditional animal lymphocyte separation solution is configured with diatrizoate and polysucrose. The principle is to rely on polysucrose to form a concentration gradient under centrifugation conditions to achieve the separation of different cells, but because the viscosity of polysucrose is very high, it is not conducive to cell movement during the separation process. In order to improve the viscosity of polysucrose, often in A certain amount of diatrizoate meglumine is added to the formula to reduce the viscosity and facilitate the movement of cells during the separation process. But the price of diatrizoate meglumine is very expensive. We try to replace diatrizoate meglumine with sodium diatrizoate, and the price will be reduced by 6 times. Our preliminary test results ...

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Problems solved by technology

But at the same time, we also found that after substituting diatrizoate meglumine with sodium diatrizoate, the formation time of cell bands during centrifugation (cell banding) was significantly prolonged during lymphocyte separation.

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