Method for assaying amphetamines, ketamine, pethidine and methadone in blood and urine
A technique for amphetamines and ketamine, applied in the fields of analytical chemistry and forensic identification, can solve the problems of cumbersome methods, time-consuming, and poor specificity of ELISA, and achieve the effect of simple pretreatment process, good accuracy, and high sensitivity
Inactive Publication Date: 2016-05-11
FUDAN UNIV
View PDF3 Cites 12 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Practice shows that the specificity of the enzyme-linked immunosorbent assay is poor, it is difficult to distinguish structural analogs, and false positives often occur. Therefore, it can only be used as a means of preliminary screening, and further gas chromatography-mass spectrometry or Liquid chromatography-mass spectrometry is used for confirmation, and before biological samples are analyzed by gas chromatography-mass spectrometry or liquid chromatography-mass spectrometry, the samples need to be extracted and purified, and this step involves extracting the liquid The process of evaporating to dry, so it often oc...
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0032] Chromatographic conditions used:
[0033] Chromatographic column: HP-5MS elastic quartz capillary gas chromatography column, 30m×0.25mm×0.25μm
[0034] Column temperature: 100°C (1.5min)—25°C / min—280°C (15min)
[0035] Solvent delay time: 3min
[0036] Injection port / transfer line temperature: 250°C / 280°C
[0037] Carrier gas: high-purity He, constant flow 1mL / min
[0038] EI source electron energy: 70Ev
[0039] Scanning method: selected ion monitoring (SIM)
[0040] Injection volume: 2μL
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more PUM
Login to view more
Abstract
The invention belongs to the technical field of analytical chemistry, and relates to a method for rapidly assaying four amphetamines (amphetamine, methamphetamine, methylene-dioxy-amphetamine and methylene-dioxy-methamphetamine), ketamine, pethidine and methadone in blood and urine. The method is high in sensitivity and specificity and wide in linear range, can be used for qualitatively and quantitatively assaying analytes of unknown concentrations in the blood and the urine, and is simple and rapid to operate; according to the method, processes of extracting, and drying by evaporating are not required, and characteristics that a judicial expertise task is urgent and has a high requirement on assaying time can be met; an assaying system, provided by the invention, provides a new technical platform for drug assaying, and is easy to popularize and apply.
Description
technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a method for measuring amphetamines, ketamine, pethidine and methadone in blood and urine. This method can be used to quickly and easily determine 4 kinds of amphetamines (amphetamine, methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine) ketamine, pethidine and methadone and other compounds in blood and urine, especially suitable for in the field of forensic identification. Background technique [0002] According to data, currently widely abused drugs include four amphetamine compounds (amphetamine (AMP), methamphetamine (MAMP), methylenedioxyamphetamine (methylenedioxyamphetamine, MDA), methylenedioxy Methamphetamine (methylenedioxymethamphetamine, MDMA), as well as ketamine and pethidine. [0003] Studies have shown that the toxicity of AMP and MAMP is mainly based on the excitatory effect of the central nervous system; MDA and MDMA have both excitato...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more Application Information
Patent Timeline
Login to view more
IPC IPC(8): G01N30/02G01N30/06
Inventor 饶渝兰张玉荣吴宗平姜宴叶永红张润生沈忆文赵子琴过林林泽彬董晓茹黄志斌
Owner FUDAN UNIV
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Try Eureka
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap