Health care food with male health care effect
A technology for health food and food, applied in the direction of food ingredients, functions of food ingredients, food ingredients containing natural extracts, etc., can solve the problems of human body side effects and obvious side effects, and achieve the promotion of testosterone secretion, no toxic side effects, and human body absorption rate. high effect
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Embodiment 1
[0017] Example 1 Polypeptide Separation
[0018] Crush Cynomorium cynomorium, and make the centrifuged residue into a suspension with water, heat the suspension at 82°C for 10 minutes, adjust the pH value to 8 after cooling, add papain, trypsin and pepsin for hydrolysis, and the hydrolysis time is 2 hours , the temperature is 50°C, the pH value is 6.5, the enzyme dosage is 1.9%, the supernatant obtained by centrifugation or filtration is a polypeptide solution, and the polypeptide solution is prepared with dextran G-50 (SephadexG-50) (1.5cm ×90cm) separation, elution with 25mmol / L HCl solution, flow rate 1.5mL / min, collect the eluted product, adjust the solution to pH7.0, centrifuge at 10000 rpm for 15 minutes, and freeze-dry the supernatant for later use; Sodium sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) recovered small molecular weight bands, and a total of 39 small peptide sequences were obtained. It has been verified that 16 small peptides have the function of ...
Embodiment 2
[0037] The preparation of embodiment 2 health food
[0038] Prepare ingredients according to the following proportions: 5 parts of wolfberry fruit, 5 parts of Tongkat Ali tree root, 3 parts of angelica, 2 parts of dried okra, 2 parts of longan meat, 2 parts of plantago seed, 4 parts of epimedium, safflower 0.5 parts, 1 part of rehmannia root, 4 parts of medlar, 25 parts of honey, 1 part of golden cherry, 2 parts of black soybean, 2 parts of Morinda officinalis, 1 part of Polygonum multiflorum, 1 part of isolated polypeptide. Among them, there are 16 types of polypeptides, as shown in sequences 1-16 respectively. Each polypeptide, together with other components, can be prepared as a food product.
[0039] The production steps are as follows:
[0040] A. Crush the root of Tongkat Ali tree, angelica, dried okra, plantain seed, epimedium, safflower, golden cherry, black bean, morinda officinalis, and Polygonum multiflorum into a coarse powder with a traditional Chinese medicine ...
Embodiment 3
[0046] Example 3 Security Verification
[0047] The food prepared in Example 2 is verified for safety. According to the conventional verification methods in the art, it can be concluded that the present invention is confirmed to be safe and non-toxic by animal experiments. The experimental results and conclusions are as follows:
[0048] (1) The acute toxicity test shows that: according to the acute toxicity dose classification standard, it belongs to the actual non-toxic level;
[0049] (2) The results of bone marrow cell micronucleus test, sperm abnormality test and Ames test are negative;
[0050] (3) 30-day feeding test: the growth condition of the experimental animals was good, and compared with the control group, hematological examination, biochemical examination, main viscera ratio and histological examination results showed no abnormality, which proved that the present invention was safe and nontoxic.
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