RCA (rolling circle amplification)-terahertz metamaterial biosensor and method for rapidly detecting multi-drug resistant tuberculosis

A technology of biosensor and rolling circle amplification, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and microorganism measurement/inspection. It can solve the problems of poor stability of results and long cycle, and achieve stable and reliable results.

Active Publication Date: 2016-05-25
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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Problems solved by technology

However, at present, RCA is mainly detected by agarose gel elect

Method used

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  • RCA (rolling circle amplification)-terahertz metamaterial biosensor and method for rapidly detecting multi-drug resistant tuberculosis
  • RCA (rolling circle amplification)-terahertz metamaterial biosensor and method for rapidly detecting multi-drug resistant tuberculosis
  • RCA (rolling circle amplification)-terahertz metamaterial biosensor and method for rapidly detecting multi-drug resistant tuberculosis

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Embodiment Construction

[0030] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. For the experimental methods not specified in the examples, the conventional conditions are generally followed, such as the conditions described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al.), or the conditions suggested by the manufacturer.

[0031]Rolling circle amplification-terahertz metamaterial biosensor, comprising a terahertz metamaterial, a capture probe coated in a reaction region of the terahertz metamaterial to identify a template sequence to be detected, and a polystyrene slide, the polystyrene slide Covering the upper layer of the terahertz metamaterial and forming a liquid film cavity for liquid phase amplification reaction, the height of the liquid film cavity is about 50 μm; the structure of the terahertz metamaterial is as follows figure 1 As shown, it is composed of metal split resonators peri...

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Abstract

The invention relates to an RCA-terahertz metamaterial biosensor and a method for rapidly detecting multi-drug resistant tuberculosis. The biosensor comprises a terahertz metamaterial, a capture probe and a polystyrene slide, wherein the capture probe is wrapped with the reaction zone of the terahertz metamaterial to identify a to-be-detected template sequence, the upper layer of the terahertz metamaterial is covered with the polystyrene slide, a liquid film cavity gap used for liquid phase amplification reaction is formed, the capture probe wrapped with the RCA-terahertz metamaterial biosensor is crossbred with an RCA circular template, in-situ replication amplification is realized, the result obtained with the method is stable and reliable, and detection of five drug resistant sites of multi-drug resistant tuberculosis can be finished in three hours.

Description

technical field [0001] The invention belongs to the detection field, and in particular relates to a rolling circle amplification-terahertz metamaterial biosensor, and also relates to a method for rapidly detecting multidrug-resistant Mycobacterium tuberculosis by the rolling circle amplification-terahertz metamaterial biosensor. Background technique [0002] In recent years, various factors such as the increase of population mobility have made the infection rate of Mycobacterium tuberculosis remain high, and tuberculosis is spreading rapidly to the world. The incidence of multi-drug resistant Mycobacterium tuberculosis is increasing due to delay in diagnosis of the disease and overuse of anti-tuberculosis drugs. Ordinary tuberculosis only needs to take first-line anti-tuberculosis drugs (such as: isoniazid, rifampicin, etc.), and adopt the principles of early, joint, appropriate, regular and full course, and it can be cured in about half a year. Mycobacterium tuberculosis i...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12Q1/68C12Q1/04C12R1/01
CPCC12Q1/682C12Q1/6825C12Q2531/125C12Q2543/101C12Q2537/162C12Q2565/607
Inventor 杨翔府伟灵黄庆罗阳刘跃平余抒徐含青赵祥刘羽杨柯黄娇祺
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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