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Spiral-capillary-based magnetic cell sorting apparatus and method

A capillary and helical technology, which is applied in the field of cell magnetic sorting devices based on helical capillaries and cell magnetic sorting devices, can solve the problems such as the inability to achieve continuous and uninterrupted sorting, and achieves reduction of sorting time and improvement of sorting. The effect of efficiency

Inactive Publication Date: 2016-06-01
ARTHRO ANDA TIANJIN BIOLOGIC TECH +1
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The magnetic separation rack has no life limit, but the sorting test tube must be replaced every time it is sorted, and continuous and uninterrupted sorting cannot be achieved

Method used

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  • Spiral-capillary-based magnetic cell sorting apparatus and method

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Embodiment Construction

[0018] The present invention will be described in further detail below in conjunction with accompanying drawing and specific embodiment, take the separation of CD3+ cells as an example:

[0019] Such as figure 1 As shown, the cell magnetic separation device based on the present invention includes: a helical capillary 3 and a ring magnet 4 . The outer diameter of the spiral capillary 3 is 1.5 mm, the inner diameter is 1.12 mm, and the diameter of the spiral outer ring is 17.8 mm. There are two ports, Fill 1 and Drain 2. The liquid filling port and the liquid discharging port are located on the upper side of the ring magnet. The material of the ring magnet is RuFeB, and the size is 18mm in inner diameter, 55mm in outer diameter, and 60mm in height. The direction of the magnetic field line of the ring magnet is distributed along the direction perpendicular to the axis in the circular hole, and the closer to the surface of the inner wall, the greater the magnetic field intensit...

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Abstract

The invention discloses a spiral-capillary-based magnetic cell sorting apparatus and method, comprising four steps: S1, feeding a sample; S2, separating in a stationary manner; S3, washing at low flow speed; S4, collecting at high flow speed. A spiral capillary placed in the center of a circular magnet is used such that all cell and magnetic bead turbid liquids are near a tube wall of the circular magnet. As the tube wall of the circular magnet has highest magnetic field strength, it is possible to make full use of a magnetic field, improve sorting efficiency and reduce sorting time by using the apparatus. After step S4 is over, the spiral capillary is fully filled with a buffer solution, it is possible to continuously carry out the step S1 of sample feeding and start a next sorting procedure such that cells are continuously sorted; the invention is applicable to sorting of a large quantity of cells.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a spiral capillary-based cell magnetic sorting device and a cell magnetic sorting device method. Background technique [0002] Cell magnetic separation technology is to use antibodies coupled to magnetic particles to identify and label target cell surface antigens with high specificity, and then use the power of a magnetic field to screen magnetic beads to remove cells that are not bound to magnetic beads and obtain purified target cells , is a common technique for cell sorting and enrichment. At present, there are two main types of cell magnetic separation products widely used, one is the magnetic separation rack, representative products such as StemCell's EasySep TM Magnet, this is a customized powerful magnet, by putting the cell suspension labeled with magnetic beads in a test tube and sticking to the surface of the magnet, and then standing still for a period of time, the cells...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12M1/24C12N5/00
CPCC12M47/04C12N5/00
Inventor 刘晓华李洲孙雪莲朱建民魏华英李召雨刘江周洪锐李昀地杨延瑞
Owner ARTHRO ANDA TIANJIN BIOLOGIC TECH
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