Anti-p185erbb2 human-mouse chimeric antibody chab26, mammary gland-specific expression vector, transgenic fvb mouse and preparation method thereof

A p185erbb2, human-mouse chimeric antibody technology, applied in the field of bioengineering, can solve the problems of high treatment costs and high production costs for tumor patients, and achieve the effects of avoiding anti-antibody reactions, low cost, and easy scale-up
CN105646704BInactive Publication Date: 2019-11-15GUANGXI MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGXI MEDICAL UNIVERSITY
Publication Date
2019-11-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an anti-p185<erbB2> human-mouse chimeric antibody ChAb26. The anti-p185<erbB2> human-mouse chimeric antibody ChAb26 is mainly composed of a heavy chain gene H and a light chain gene L, and the heavy chain gene H and the light chain gene L are respectively connected to a mammary gland specific expression plasmid pBC1 in order to obtain p185<erbB2> human-mouse chimeric antibody ChAb26 mammary gland specific expression vectors pBC1-H and pBC1-L. The pBC1-H and pBC1-L are linearized, the linearized pBC1-H and pBC1-L are introduced to FVB mouse zygotes to produce a transgenic FVB mouse, and the anti-p185<erbB2> human-mouse chimeric antibody ChAb26 can be obtained through mammary gland specific expression with the transgenic FVB mouse as a mammary gland bioreactor. The antibody reduces the immunogenicity of the v monoclonal antibody, and a medicinal recombinant protein produced through the transgenic FVB mouse has the advantages of high output, low cost, high activity, easy large-scale formation, shortening of the manufacturing period of new medicines, and huge market potential.
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Description

technical field

[0001] The technology of the invention belongs to the field of bioengineering, in particular to an anti-p185 erbB2 Human-mouse chimeric antibody ChAb26, mammary gland-specific expression vector, transgenic FVB mouse and preparation method thereof. Background technique

[0002] Shih et al. (1981) first discovered a new proto-oncogene in rat embryonic neuroblasts and named it the neu gene. Subsequently, the neu proto-oncogene and the proto-oncogene erb B were found to be highly homologous through nucleic acid sequence analysis comparison and chromosome profile analysis. Therefore, the neu proto-oncogene was considered a related gene of the erb B proto-oncogene and was named erb B2 / HER2. Coussens et al. found that the human proto-oncogene erb B2 is located in the 2 region 1 band (17q21) of the long arm of human chromosome 17, and its encoded product is a receptor tyrosine kinase (RTK, receptor tyrosine kinase) with a molecular weight of 185 kDa, so it is also ...

Claims

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