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A kind of preparation method of self-adhesive skin repair hydrogel

A skin repair and hydrogel technology, applied in capsule delivery, pharmaceutical formula, nanocapsules, etc., can solve problems such as insufficient skin donor sites, and achieve high efficacy, avoid drug resistance, and good adhesion

Active Publication Date: 2018-08-07
CHENGDU JIAODA MEDICAL SCI & TECH COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for various diseases such as extensive burns, sufficient skin is required for repair, and there is often a problem of insufficient skin donor sites

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The quaternized chitosan adopts prior art, and its preparation steps are as follows:

[0022] 1. Prepare chitosan solution: dissolve chitosan in acetic acid solution containing 2%-5% mass fraction (chitosan concentration: 1 wt.%-10 wt.%)

[0023] 2. Prepare glycidyltrimethylammonium chloride solution: dissolve 30 g glycidyltrimethylammonium chloride (ETA) in 30ml water, then add the ETA solution dropwise to the CS solution, and stir magnetically.

[0024] 3. React the mixed solution at 80°C for 10 h, then pour it into cold acetone, place it in the refrigerator for 24 h, then pour out the acetone, leaving a gel-like product.

[0025] 4. Dissolve the product in 100 ml methanol, and then pour it into a mixed solution of 250 ml ethanol: acetone (volume ratio 1:4) to precipitate the product.

[0026] 5. The product was dialyzed with deionized water at room temperature, and freeze-dried to obtain quaternized chitosan powder.

[0027] B. Preparation of dopamine-functionalize...

Embodiment 2

[0034] A. quaternization chitosan is with step A in example 1.

[0035] B. Preparation of dopamine-functionalized gelatin

[0036] Gelatin and dopamine were added to 200 ml of Tris buffer (10 mM, pH=8.5), wherein the mass ratio of gelatin to dopamine was 3:1, and the mixed solution was sonicated in an ice bath to disperse. The mixture was vigorously stirred overnight with heating. After the dopamine and gelatin fully react, the solution is filtered, washed, dialyzed and freeze-dried to obtain dopamine-functionalized gelatin.

[0037] C. Preparation of Bovine Serum Albumin (BSA) Nanoparticles Loaded with Epidermal Growth Factor (EGF)

[0038] Dissolve epidermal growth factor (EGF) which can promote skin regeneration in 20 mg / ml BSA solution. Slowly and uniformly add ethanol dropwise to the mixed solution of BSA and growth factors (volume ratio of ethanol-BSA solution is 1:4), and continue to stir for 24 hours to obtain nanoparticles, and finally obtain encapsulated epidermal...

Embodiment 3

[0042] A. quaternization chitosan is with step A in example 1.

[0043] B. Preparation of dopamine-functionalized gelatin

[0044] Gelatin and dopamine were added to 200 ml of Tris buffer (10 mM, pH=8.5), wherein the mass ratio of gelatin to dopamine was 4:1, and the mixed solution was sonicated in an ice bath to disperse. The mixture was vigorously stirred overnight with heating. After the dopamine and gelatin fully react, the solution is filtered, washed, dialyzed and freeze-dried to obtain dopamine-functionalized gelatin.

[0045] C. Preparation of Bovine Serum Albumin (BSA) Nanoparticles Loaded with Vascular Endothelial Growth Factor (VEGF)

[0046]Dissolve vascular endothelial growth factor (VEGF) which can promote skin regeneration in 40 mg / ml BSA solution. Ethanol was slowly and uniformly added dropwise to the mixed solution of BSA and growth factors (the volume ratio of the ethanol-BSA solution was 1:4), and the stirring was continued for 24 hours to obtain nanopart...

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PUM

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Abstract

The invention discloses a preparation method of self-adhesive skin repairing hydrogel. The preparation method includes subjecting natural chitosan to quaternary amine modification to enable the natural chitosan to have excellent bactericidal performance; preparing BSA (bull serum albumin) nano-particles embedded with growth factors promoting skin regeneration through desolvation; grafting dopamine into a natural gelatin material; crosslinking the quaternary amine modified chitosan, the growth factor embedded BAS nano-particles and the dopamine grafted gelatin to form a hydrogel material. The hydrogel material has multiple functions of adhesion, bacteria inhibition and skin wound healing promotion, thereby being used for skin repairing.

Description

technical field [0001] The invention belongs to the field of bioengineering materials, especially skin repair materials. Background technique [0002] The skin is the largest organ of the human body. A large area of ​​skin tissue exposed to the external environment may be damaged at all times. Burns, mechanical trauma, and ulcers caused by chronic diseases (such as diabetes) are the main causes of skin defects and loss of function. Extensive burns, chronic skin ulcers, skin cancer, diabetic ulcers, bedsores, etc. can often be treated with skin grafts. However, for various diseases such as extensive burns, sufficient skin is required for repair, and there is often a problem of insufficient skin donor sites. Therefore, it is of great significance to develop skin repair products that can effectively promote wound healing and skin tissue regeneration. [0003] The primary function of the skin is to isolate body tissues from the external environment and act as a protective barr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L26/00
CPCA61L26/0023A61L26/0038A61L26/0066A61L26/008A61L2300/204A61L2300/208A61L2300/404A61L2300/414A61L2300/624C08L89/00C08L5/08
Inventor 鲁雄
Owner CHENGDU JIAODA MEDICAL SCI & TECH COMPANY
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