Parallel plate test chamber device for loading flow shear stress in cell culture
A technology of cell culture and parallel plates, which is applied in the field of biomedicine, can solve problems such as theoretical errors of fluid shear stress, and achieve the effects of reducing thickness accuracy requirements, reducing costs, and improving control accuracy
Inactive Publication Date: 2016-06-15
郭菲
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Problems solved by technology
[0003] In order to solve the problem of using a parallel plate experiment chamber with replaceable culture slices, to avoid the theoretical error of fluid shear stress caused by the size error of the culture slices when the culture slices are replaced, and to avoid occupying too much culture area on the culture slices, the present invention Provides a technical solution for a parallel plate experimental chamber device for loading fluid shear stress in cell culture
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[0033]A parallel plate experimental chamber device for loading fluid shear stress for cell culture, including an upper chamber (1), a lower chamber (2), and an upper chamber (1) with Upper window (3), upper and lower window (4) on the lower cavity (2), culture sheet (5), sealing gasket (6), joint (7), sealing ring (8), elastic pad (9), bolts (10) Composition, the combined cavity is connected to the outside world through joints (7) at four positions of the four-way interface (a), (b), (c), and (d); the upper and lower cavities are connected by bolts Fixed; all parts of the experimental chamber can withstand high temperature disinfection, and the opposite side of the cell crawling surface culture sheet (5) uses an elastic pad (9) to form a reverse force on the culture sheet so that the upper chamber (1) and the lower chamber After the body (2) is combined, the culture sheet (5) is completely attached to the flow channel of the upper cavity (1), and the upper cavity (1) is made o...
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Abstract
The invention belongs to the field of flow shear stress and provides a parallel plate test chamber device which is used for loading flow shear stress in cell culture and can conduct high-temperature sterilization repeatedly, through reactive force of an elastic pad, a culture piece and a laminar flow channel can be tightly attached, input controllable liquid flow is converted into controllable liquid laminar flow, when the culture piece is replaced, influence on the control precision of liquid laminar flow control is greatly lowered, and cell culture can be achieved on the area except a pressing edge of the culture piece. Compared with other schemes, under the same culture piece, the repeated culture effect of obtaining a larger culture area or better flow shear stress control is achieved.
Description
technical field [0001] The invention belongs to the field of biomedicine, and relates to a parallel plate experimental cavity device used in experiments for loading fluid shear stress in cell culture, providing a laminar flow and forming a stable fluid shear stress stimulus for loading cultures such as cells The technical scheme of the experimental chamber device. Background technique [0002] The current fluid shear stress value is mainly calculated by theoretical formulas. Therefore, in order to obtain the required fluid shear stress value, the parameters of the parallel plate experimental chamber are controlled to obtain the theoretical fluid shear stress. Under the premise of satisfying the Reynolds system Re=ρQD / (μwh), where ρ is the liquid density and D is 4wh / [2*(w+h)], the shear stress calculation formula is: τ=6μQ / (wh^2) where τ is the shear stress value of the fluid, μ is the viscosity of the liquid, Q is the flow rate of the liquid, w is the width of the flow cha...
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IPC IPC(8): C12M3/04C12M1/00
CPCC12M23/04C12M23/34C12M23/46
Inventor 郭菲
Owner 郭菲