Measuring device and quantitative assessment method of retinal photic injury

Abstract

The invention provides a measuring device for quantatively assessing photic injury of retinal pigment epithelial cells due to a light source and a quantitative assessment method based on the measuring device.The method enables quick, efficient and accurate assessment on quantitative indexes of retinal photic injury.The measuring device can provide stable testing environment and testing conditions, and it is possible to accurately and effectively assess photic injury degree of retinal pigment epithelial cells due to the light source.The problem that results of evaluation by the use of an existing retinal pigment epithelial cell line, such as presence or absence of photic injury, have the major problems with repeatability and accuracy is effectively solved, so that data support is provided for the research and development and scientific research of related lighting products.

Description

technical field [0001] The invention belongs to the field of biochemical detection, and in particular relates to a measuring device and a measuring method for quantitatively evaluating light damage of a light source to retinal pigment epithelial cells. Background technique [0002] In order to evaluate the safety of different light sources on the human retina, a subjective feedback survey or a large number of photodamage tests on individual animals are usually used. In these tests, the stimulation of human eyes and tissue damage caused by light are collectively referred to as photochemical damage tests. [0003] In the past 40 years of research, many scholars have used various experimental animals (mainly small animals) to conduct a large number of researches on retinal photodamage and its mechanism. As the most widely used method among photodamage test methods, for example, high-intensity light exposure or continuous light damage to small animals is known. The test method...

AI Technical Summary

Problems solved by technology

Some studies have used primary cultured animal retinal photoreceptor cells, but these cells cannot be stably expanded during in vitro culture, so the reproducibility of the experiment is poor; some studies have used retinal pigment epithelial cell lines, but this type of cells The physiological characteristics of cells are completely different at different stages of culture, so the results of different studies are also very different

[0007] At present, the use of retinal pigment epithelial cell lines to evaluate the presence or absence of photodamage has major problems in terms of repeatability and accuracy, and in terms of actually predicting the safety of the human retina, this method is not always sufficient. Satisfactory, people expect to develop a stable, high-precision in vitro photochemical damage test device and method

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