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Optimized dosage of sodium arsenite for promoting synthesis and secretion of insulin in pancreatic beta cells

A technology of sodium arsenite and β cells, which can be applied to medical preparations containing active ingredients, inorganic active ingredients, endocrine system diseases, etc. It can solve the problem that spiced tofu is difficult to preserve thick juice and taste, there is no long-term preservation method, spiced tofu, etc. Problems such as short shelf life of tofu

Inactive Publication Date: 2016-06-22
陈慧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The five-spice tofu sold in the market has a short shelf life, and the five-spice tofu in vacuum compression packaging is difficult to preserve its original thick juice and taste. As a kind of food that is loved by people, there is no proper way to preserve it for a long time

Method used

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Embodiment Construction

[0011] The following examples of preparation are listed to further describe the present invention in detail, and these examples are only used in the present invention and do not limit the present invention.

[0012] The experimental steps of this embodiment are as follows:

[0013] 1. NIT-1 cell culture

[0014] 1. Preparation of low-sugar DMEM medium

[0015] (1) Pour 12.5g of low-sugar DMEM powder into a large beaker, add 850mlddH 2 O stirring;

[0016] (2) Add appropriate amount of NaHCO 3 , so that the pH value is adjusted to 7.2-7.4;

[0017] (3) Continue to add ddH 2 O, dilute to 1000ml, stir until completely dissolved;

[0018] (4) Filter through a 0.22 μm microporous filter, aliquot and store at 4°C.

[0019] 2. NIT-1 cell culture and passage

[0020] (1) The NIT-1 cell line was routinely cultured in low-glucose DMEM medium containing 10% FBS;

[0021] (2) When the cell growth density is >80%, digest with 0.25% trypsin at 37°C for 1-3min, observe under the mic...

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Abstract

On the basis of studies on influence the sodium arsenite on the synthesis and secretion of insulin in pancreatic beta cells, the invention provides an optimized dosage of the sodium arsenite for promoting the synthesis and the secretion of the insulin in the pancreatic beta cells. The optimized dosage of the sodium arsenite for promoting the synthesis and the secretion of the insulin in the pancreatic beta cells is obtained from various experiments, so that the unhelpful influence of arsenic in conventional dosages on human body is changed; the proliferation of insulin cells is promoted by controlling the amount of the sodium arsenite, and the number of the insulin cells is directly linked to the treatment of such diseases as diabetics; therefore, the invention has a relatively important value on the study.

Description

technical field [0001] The present invention specifically relates to the optimization of the dosage of sodium arsenite for promoting insulin synthesis and secretion of islet beta cells. Background technique [0002] Tofu is loved by people for its high protein and easy digestion and absorption. There are many processing methods for tofu cooked food products. According to the types of seasonings and processing methods used, products with different flavors and characteristics can be formed. The five-spice tofu sold in the market has a short shelf life, and the five-spice tofu in vacuum compression packaging is difficult to preserve its original thick juice and mouthfeel. [0003] Canned food is more convenient to eat. It is exhausted and sealed to isolate the food in the can from the outside world, avoiding the influence of external conditions on the food, preventing the action of microorganisms in the air, and facilitating long-term storage of food. After the cans are sealed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/36A61P5/48
Inventor 陈慧
Owner 陈慧
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