Mini-type grafting method of Koelreuteria bipinnata var. integrifoliola 'Jinyan'

A golden flame color luan, micro-grafting technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of affecting sub-generation reproduction, large environmental impact, difficulty in reproduction, etc., to improve the survival rate and germination. rate effect

Inactive Publication Date: 2016-07-06
JIANGSU ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Jinyan Cailuan (Koelreuteriabipinnatavar.integrifoliola'Jinyan') is a healthy growing variety selected from Huangshan eucalyptus. Its main characteristics are that the trunk or branches are yellow, the new buds and leaves are orange-red in spring, the summer leaves are yellow-green, and the autumn leaves are golden yellow. It has high horticultural ornamental value. The plant is mainly propagated by grafting at present. It is greatly affected by the environment and has a low survival rate. In subculture, the leaves of test tube sprouts will have the problem of abscission and shedding, which will seriously affect further subculture

Method used

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  • Mini-type grafting method of Koelreuteria bipinnata var. integrifoliola 'Jinyan'
  • Mini-type grafting method of Koelreuteria bipinnata var. integrifoliola 'Jinyan'

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Experimental program
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Effect test

Embodiment 1

[0018] The rootstock culture is to use Huangshan eucalyptus to grow and grow healthy single plant tissue culture. Before the tissue culture, the dust on the surface of the material is cleaned, and then cut into 1-1.5cm stem segments with lateral buds, and the surface is sterilized. When sterilizing, first use 75 Soak in % alcohol for about 10 seconds, rinse twice with sterile water, then sterilize with 0.1% HgCl for 5-6 minutes, and then rinse with sterile water for 3 times. The rootstock is selected to be cultivated for 15 days, and the germination zone 1 is selected after tissue culture , 2, 3, and 4 leaf shoots are used as rootstock grafting, and the medium is 1 / 2MS+6-BA0.02mg / L+NAA0.05mg / L++IAA0.05mg / L+IBA0.02mg / L+sucrose 30g / L; for scion culture, inoculate the shoots of Jinyancailuan seedlings in mid-April to inoculate the tissue culture buds, and the medium is 1 / 2MS+6-BA0.02mg / L+NAA0.05mg / L++IAA0.05mg / L + IBA0.02mg / L+ sucrose 30g / L, the cultivation time is 15 days, the ...

Embodiment 2

[0020] The rootstock culture is to use Huangshan eucalyptus to grow and grow healthy single plant tissue culture. Before the tissue culture, the dust on the surface of the material is cleaned, and then cut into 1-1.5cm stem segments with lateral buds, and the surface is sterilized. When sterilizing, first use 75 Soak in % alcohol for about 10S, rinse twice with sterile water, then sterilize with 0.1% HgCl for 5-6 minutes, and then rinse with sterile water for 3 times. The shoots of 2, 3, and 4 leaves are used as rootstock grafting, and the medium is 1 / 2MS+6-BA0.02mg / L+NAA0.05mg / L++IAA0.05mg / L+IBA0.02mg / L+sucrose 30g / L; scion culture inoculates tissue culture buds with tender shoots of Jinyan Cailuan seedlings in mid-April, and the medium is improved formulation 2 1 / 2MS+6-BA0.02mg / L+NAA0.05mg / L++IAA0.05mg / L+IBA0.02mg / L+sucrose 30g / L, the cultivation time is 15 days, the dust on the surface of the material is cleaned before tissue culture, and then cut into 1-1.5cm stem segmen...

Embodiment 3

[0022]The rootstock culture is to use Huangshan eucalyptus to grow and grow healthy single plant tissue culture. Before the tissue culture, the dust on the surface of the material is cleaned, and then cut into 1-1.5cm stem segments with lateral buds, and the surface is sterilized. When sterilizing, first use 75 Soak in % alcohol for about 10 seconds, rinse twice with sterile water, then sterilize with 0.1% HgCl for 5-6 minutes, and then rinse with sterile water for 3 times. The rootstock is selected to be cultivated for 15 days. , 2, 3, and 4 leaf shoots are used as rootstock grafting, and the medium is an improved dosage form-1 / 2MS+6-BA0.02mg / L+NAA0.05mg / L++IAA0.05mg / L+IBA0.02mg / L + sucrose 30g / L; the scion was inoculated with the tender shoots of Jinyan Cailuan seedlings in mid-April, and the medium was improved formulation 2 1 / 2MS+6-BA0.02mg / L+NAA0.05mg / L++ IAA0.05mg / L+IBA0.02mg / L+sucrose 30g / L, the cultivation time is 15 days, the dust on the surface of the material is cl...

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Abstract

The invention relates to a mini-type grafting method of Koelreuteria bipinnata var. integrifoliola 'Jinyan'. The method mainly comprises the following steps: stock culture, scion culture, stock selection, scion selection, test tube grafting and culture after grafting. A healthy single tissue culture bud seedling of Huangshan Koelreuteria Paniculata laxm is used as a stock and is cultured in a culture medium for 15-45 days; a bud seedling formed by inoculation and tissue culture of a seedling tender bud of Koelreuteria bipinnata var. integrifoliola 'Jinyan' as a scion and is cultured in a culture medium for 15-45 days; a test tube grafting manner adopts a cleft grafting method; culture conditions of the stock culture, the scion culture and the culture after grafting are as follows: in different improved culture media of 1 / 2MS+0.02mg / L 6-BA+(0.01-0.05)mg / L NAA+(0.03-0.08)mg / L IAA+(0.02-0.06)mg / L IBA+(20-40)g / L sucrose, the illumination intensity is 2500lx, the temperature condition is 25-27 DEG C and the illumination period is 12 hours; and after the grafting manner is used, the survival rate and the germination rate are both 88% and above.

Description

technical field [0001] The invention relates to a micro-grafting method of whole-leaved eucalyptus, in particular to a micro-grafting method of golden-flame-colored eucalyptus. Background technique [0002] Micrografting is a combination of plant tissue culture and grafting techniques. Micro-grafting, also known as test-tube grafting, is a technique of grafting rootstock and scion in a test tube to obtain new plants. It is based on tissue culture, and the grafted plants are cultivated under artificially controlled environmental conditions, eliminating the influence of external environmental factors on the survival of grafting. influences. [0003] Jinyan Cailuan (Koelreuteriabipinnatavar.integrifoliola'Jinyan') is a healthy growing variety selected from Huangshan eucalyptus. Its main characteristics are that the trunk or branches are yellow, the new buds and leaves are orange-red in spring, the summer leaves are yellow-green, and the autumn leaves are golden yellow. It has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/005
Inventor 蒋泽平张敏黄利斌
Owner JIANGSU ACAD OF FORESTRY
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