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Dairy cow sperm cryopreservation method

A preservation method and low-temperature freezing technology, which is applied in the field of low-temperature cryopreservation of dairy cow sperm, can solve problems such as sperm death, and achieve the effects of easy promotion, improved freezing effect, and good protection effect

Inactive Publication Date: 2016-07-06
云南中科胚胎工程生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] What the present invention aims to solve is that the existing dairy cow sperm uses egg yolk as an additive. Although the freezing process is stable, a large number of sperm will die after recovery. It provides a dairy cow sperm that has a protective effect on the freezing of dairy cow sperm and can improve the freezing effect. Low temperature cryopreservation solution and preservation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: A method for low-temperature cryopreservation of dairy cow sperm. The dairy cow sperm is placed in a low-temperature cryopreservation solution added with dove egg yolk for low-temperature preservation, and the protective effect of low-density lipoprotein contained in the dove egg yolk is used for dairy cow sperm. , to reduce the mortality rate of cow sperm after recovery; the specific cryopreservation steps are as follows:

[0027] (1) Take 15 μL of semen and put it into a test tube, then add 75 μL of preservation solution A to the test tube for dilution;

[0028] (2) Put the mixture obtained in step (1) in an ice bath at 4°C for 1 hour, then take 75 μL of preservation solution B, pre-cool to 4°C, divide into five equal portions, each 15 μL, and add in five times In the mixture, the time interval between each addition is 6 minutes, and finally placed in an ice bath at 4°C for 30 minutes;

[0029] (3) Take 0.2ml of the sperm mixture obtained in step (2) an...

Embodiment 2

[0036] Embodiment 2: A method for low-temperature cryopreservation of dairy cow sperm. Putting dairy cow sperm into a low-temperature cryopreservation solution added with dove egg yolk for low-temperature storage, utilizing the protective effect of low-density lipoprotein contained in dove egg yolk on dairy cow sperm , to reduce the mortality rate of cow sperm after recovery; the specific preservation steps are as follows:

[0037] (1) Take 20 μL of semen and put it into a test tube, then add 100 μL of preservation solution A to the test tube for dilution;

[0038] (2) Put the mixture obtained in step (1) in an ice bath at 4°C for 1 hour, then take 100 μL of preservation solution B, pre-cool it to 4°C, divide it into five equal portions, each portion is 20 μL, and divide it into five equal portions. Add to the mixed solution once, the time interval between each addition is 6 minutes, and finally place it in an ice bath at 4°C for 30 minutes;

[0039] (3) Take 0.2ml of the spe...

Embodiment 3

[0046] Embodiment 3: A method for low-temperature cryopreservation of dairy cow sperm, in which dairy cow sperm are placed in a low-temperature cryopreservation solution added with dove egg yolk for low-temperature preservation, and the protective effect of low-density lipoprotein contained in dove egg yolk on dairy cow sperm is utilized , to reduce the mortality rate of cow sperm after recovery; the specific preservation steps are as follows:

[0047] (1) Take 17 μL of semen and put it into a test tube, then add 85 μL of preservation solution A to the test tube for dilution;

[0048] (2) Put the mixture obtained in step (1) in an ice bath at 4°C for 1 hour, then take 85 μL of preservation solution B, pre-cool it to 4°C, and divide it into five equal parts, each with 17 μL, and divide it five times Add to the mixture, the time interval between each addition is 6 minutes, and finally place in an ice bath at 4°C for 30 minutes;

[0049] (3) Take 0.2ml of the sperm mixture obtai...

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Abstract

A dairy cow sperm cryopreservation method relates to sperm preservation, in particular to a milk cow sperm cryopreservation method which has a protective effect on dairy cow sperm freezing and can improve the freezing effect. The cow sperm cryopreservation method is characterized in that the preservation method is to put the cow sperm into a low-temperature cryopreservation solution added with dove egg yolk for low-temperature preservation, and utilize the low-density lipoprotein contained in the dove egg yolk to inhibit the cow sperm Protective effect, reducing mortality in dairy cows after semen recovery. The dairy cow sperm cryopreservation method of the present invention adopts pigeon egg yolk to prepare, and pigeon egg yolk has a better protective effect on dairy cow sperm. Under this cryopreservation solution, dairy cow frozen sperm recovery motility, motility recovery rate and sperm quality The membrane integrity rate is higher than other cryopreservation solutions that have been reported to add different species of yolk; the use of this preservation solution can improve the freezing effect of dairy cow semen, and it is easy to popularize in practical production.

Description

technical field [0001] The invention relates to sperm preservation, in particular to a dairy cow sperm cryopreservation method which has a protective effect on the freezing of dairy cow sperm and can improve the freezing effect. Background technique [0002] The ultra-low temperature cryopreservation technology of sperm has laid a foundation for the breeding and improvement of livestock breeds, cherishing, germplasm preservation of endangered animals, and treatment of human infertility. It has been known for more than 200 years that the sperm of some mammals can survive brief exposure to slightly lower temperatures. Since 1951, when calves were obtained by artificial insemination with frozen and revived dairy cow sperm for the first time in the world, dairy cow sperm freezing technology has been very mature and widely used. In the freezing process of dairy cow sperm, in order to reduce the damage caused by freezing, some substances need to be added, such as egg yolk, buffer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 苏雷苏宇飞王姝瑭
Owner 云南中科胚胎工程生物技术有限公司
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