Preparation method and application of bis-azide-substituted polytetraethylene glycol
A polytetraethylene glycol and diazide technology is applied in the fields of anti-inflammatory, diazide-substituted polytetraethylene glycol compound and its preparation, promoting wound healing, and sterilization, so as to promote expression, improve healing effect, The effect of promoting proliferation and migration
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Embodiment 1
[0047] Example 1 Synthesis process of diazide substituted polytetraethylene glycol and nuclear magnetic detection results
[0048] 1. Synthesis of diazide-substituted polytetraethylene glycol
[0049] 1.1 Synthetic reaction route:
[0050] The reaction route is as follows:
[0051]
[0052] The preparation method is: take 40mL of tetrahydrofuran in N 2 10.156 g of polyethylene tetraethylene glycol was added under protection, and then 9 mL of methanesulfonyl chloride was added with a syringe and stirred. Under ice bath conditions, 16.2 mL of triethylamine and 10 mL of tetrahydrofuran were mixed and added dropwise to the flask, followed by a yellow-white precipitate. After 1 hour the ice bath was removed and the mixture was stirred for 3.5 hours. Withdraw dropwise and N 2 Protect the device, add 24.4mL of water to dissolve, and form two liquid phases. After cooling on ice bath, add NaHCO 3 Adjust pH≥8, then add 6.976g NaN 3 , refluxed for 24 hours. Finally, the desired...
experiment example 1
[0055] Experimental example 1 Establishment of mouse wound model and pharmacodynamics test of diazide-substituted PEG
[0056] 1. Establishment of mouse wound model and drug treatment
[0057] 1.1 Establishment of mouse wound model
[0058] Twelve mice were randomly divided into 3 cages with 4 mice per cage, numbered 1-3. After each mouse was anesthetized with 10% chloral hydrate at a dose of 0.3 mL / 100 g, the hair on the back of the mouse was removed with a shaver, and the back skin was wiped with 70% ethanol to create two wounds, respectively, and hemostatic cotton was used to stop bleeding. Salt water wipe, that is, the modeling is successful.
[0059] 1.2 Administration of wound healing mice
[0060] After modeling, mice were dosed. The administration method was external use, and 1% gelatin and diazide-substituted polytetraethylene glycol gelatin solution were applied to the two wounds of each mouse. And the wounds of the mice were observed and photographed every day....
experiment example 2
[0068] Experimental Example 2 The effect of diazide-substituted PEG on the proliferation and migration of mouse fibroblasts (NIH-3T3) and human immortalized epidermal cells (HaCaT)
[0069] The effect of diazide-substituted polyethylene tetraethylene glycol on the growth of mouse fibroblasts (NIH-3T3) and human immortalized epidermal cells (HaCaT) by MTT colorimetry
[0070] Method steps:
[0071] (1) Cell recovery and culture
[0072] The cryopreserved NIH-3T3 cells and HaCaT cells were taken out from liquid nitrogen, thawed into a petri dish, and placed at 37°C, 5% CO. 2 , cultured under conditions of saturated humidity.
[0073] (2) MTT assay to determine the effect of drugs on cell proliferation
[0074] Adherent cells in logarithmic growth phase were trypsinized and seeded on 96-well culture plates at 5000 cells per well. Placed in a 37°C, 5% carbon dioxide incubator overnight for 24 hours to allow cells to adhere. After 24 hours, the culture medium was discarded, an...
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