Method for identifying animal blood on basis of near-infrared spectrum technologies and application of method
A near-infrared spectroscopy and near-infrared technology, which is applied in the field of blood product adulteration identification, detection and analysis, can solve the problems of high experimental technical requirements, cumbersome experimental steps, environmental pollution, etc., and achieves elimination of background drift, good display effect, and reduced interference. Effect
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Embodiment 1
[0050] At present, the method of identifying animal blood is mainly the PCR technology using molecular biological means, such as research on DNA extraction methods of animal-derived food duck blood and pig blood, and double PCR detection. The method is to use chloroform-sodium acetate (NaAc) extraction method and KI extraction method to extract DNA from solid block duck blood, and to synthesize and amplify duck-derived and pig-derived component primers, and perform double PCR amplification to obtain duck and The purpose of the pig blood is to extend the band, so as to determine whether the animal-derived blood sample contains pig or duck blood components.
[0051] The instrument investment of this method has EppenDorf brand PCR instrument about 60,000-200,000 yuan; Beijing Liuyi electrophoresis instrument (with electrophoresis tank) is about 4,000 yuan; system) about 100,000-150,000 yuan; TGL-16B desktop high-speed centrifuge is about 3,000 yuan. The cost of sample preparatio...
Embodiment 2
[0055] Fisher linear discriminant analysis of embodiment 2.4 kinds of typical blood samples
[0056] This embodiment provides a method for identifying animal blood based on near-infrared spectroscopy, the main contents of which are as follows:
[0057] 1) Collect the original spectra of different blood products and perform spectral data preprocessing:
[0058] ASelect representative animal blood samples, and take four different varieties of duck blood, cow blood, pig blood and chicken blood;
[0059] B Spread the obtained blood samples evenly in a sample cup with a diameter of 75 mm, and use a near-infrared analyzer to scan 60 times with a resolution of 5 nm, and the spectral scanning range is 950-1650 nm. The sample requires reloading during scanning to maintain the uniformity of sample loading and obtain the average original spectrum of the sample.
[0060] C performs the following spectral preprocessing on the scanned near-infrared spectral data:
[0061] ①First-order de...
Embodiment 3
[0071] Embodiment 3: the identification test of adulterated blood sample
[0072] Using the modeling method in Example 2, two kinds of duck blood samples with different adulteration rates were used for differential analysis, and the blood samples used for the adulterated samples were duck blood samples and bovine blood samples.
[0073] Methods as below:
[0074] 1) Collect the original spectra of 4 standard blood products and two samples with different adulteration ratios and perform spectral data preprocessing:
[0075] A Select fresh duck blood, cow blood, pig blood and chicken blood samples, represented by numbers 1, 2, 3 and 4 respectively. In addition, two mixed blood samples of duck blood and cow blood with different adulteration ratios were prepared. The adulteration ratios were: 40% duck blood + 60% cow blood, 20% duck blood + 80% cow blood, a total of six blood samples;
[0076] B Spread the obtained blood samples evenly in a sample cup with a diameter of 75 mm, an...
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