Method for identifying animal blood on basis of near-infrared spectrum technologies and application of method

A near-infrared spectroscopy and near-infrared technology, which is applied in the field of blood product adulteration identification, detection and analysis, can solve the problems of high experimental technical requirements, cumbersome experimental steps, environmental pollution, etc., and achieves elimination of background drift, good display effect, and reduced interference. Effect

Active Publication Date: 2016-07-06
QIQIHAR UNIVERSITY
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Problems solved by technology

[0004] At present, the technology for detecting blood adulteration is only the detection method of molecular biology. The PCR technology based on DNA is widely used to identify the animal source components in food. Although the method has high accuracy, the equipment is expensive and the operator's experiment

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  • Method for identifying animal blood on basis of near-infrared spectrum technologies and application of method
  • Method for identifying animal blood on basis of near-infrared spectrum technologies and application of method
  • Method for identifying animal blood on basis of near-infrared spectrum technologies and application of method

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Experimental program
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Effect test

Embodiment 1

[0050] At present, the method of identifying animal blood is mainly the PCR technology using molecular biological means, such as research on DNA extraction methods of animal-derived food duck blood and pig blood, and double PCR detection. The method is to use chloroform-sodium acetate (NaAc) extraction method and KI extraction method to extract DNA from solid block duck blood, and to synthesize and amplify duck-derived and pig-derived component primers, and perform double PCR amplification to obtain duck and The purpose of the pig blood is to extend the band, so as to determine whether the animal-derived blood sample contains pig or duck blood components.

[0051] The instrument investment of this method has EppenDorf brand PCR instrument about 60,000-200,000 yuan; Beijing Liuyi electrophoresis instrument (with electrophoresis tank) is about 4,000 yuan; system) about 100,000-150,000 yuan; TGL-16B desktop high-speed centrifuge is about 3,000 yuan. The cost of sample preparatio...

Embodiment 2

[0055] Fisher linear discriminant analysis of embodiment 2.4 kinds of typical blood samples

[0056] This embodiment provides a method for identifying animal blood based on near-infrared spectroscopy, the main contents of which are as follows:

[0057] 1) Collect the original spectra of different blood products and perform spectral data preprocessing:

[0058] ASelect representative animal blood samples, and take four different varieties of duck blood, cow blood, pig blood and chicken blood;

[0059] B Spread the obtained blood samples evenly in a sample cup with a diameter of 75 mm, and use a near-infrared analyzer to scan 60 times with a resolution of 5 nm, and the spectral scanning range is 950-1650 nm. The sample requires reloading during scanning to maintain the uniformity of sample loading and obtain the average original spectrum of the sample.

[0060] C performs the following spectral preprocessing on the scanned near-infrared spectral data:

[0061] ①First-order de...

Embodiment 3

[0071] Embodiment 3: the identification test of adulterated blood sample

[0072] Using the modeling method in Example 2, two kinds of duck blood samples with different adulteration rates were used for differential analysis, and the blood samples used for the adulterated samples were duck blood samples and bovine blood samples.

[0073] Methods as below:

[0074] 1) Collect the original spectra of 4 standard blood products and two samples with different adulteration ratios and perform spectral data preprocessing:

[0075] A Select fresh duck blood, cow blood, pig blood and chicken blood samples, represented by numbers 1, 2, 3 and 4 respectively. In addition, two mixed blood samples of duck blood and cow blood with different adulteration ratios were prepared. The adulteration ratios were: 40% duck blood + 60% cow blood, 20% duck blood + 80% cow blood, a total of six blood samples;

[0076] B Spread the obtained blood samples evenly in a sample cup with a diameter of 75 mm, an...

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Abstract

The invention discloses a method for identifying animal blood on the basis of near-infrared spectrum technologies and application of the method, and belongs to the field of technologies for identifying, detecting and analyzing adulteration of blood products. The method includes respectively scanning pure animal blood standard samples and animal blood samples to be subjected to adulteration examination to acquire original spectra of the pure animal blood standard samples and the animal blood samples by the aid of near-infrared analyzers; carrying out spectral pre-processing on near-infrared spectral data obtained by means of scanning; extracting effective factor numbers by means of factor analysis; establishing Fisher linear discriminant analysis models by the aid of selected effective factors; carrying out linear discriminant analysis on the to-be-examined samples by the aid of Fisher linear discriminant functions of the pure animal blood standard samples to obtain prediction classification results and images; comparing the images of the pure animal blood standard samples and the to-be-examined animal blood samples to one another and identifying the to-be-examined blood samples so as to identify whether the to-be-examined samples are adulterated or not. The method and the application have the advantages that the method is high in identification accuracy, the correct discriminant rate of different varieties of blood samples can reach 100%, and the method is applicable to identifying animal blood products.

Description

technical field [0001] The invention relates to a method and application for identifying animal blood based on near-infrared spectroscopy, and belongs to the technical field of identification, detection and analysis of adulteration of blood products. Background technique [0002] Due to the large difference in price of different types of animal blood products, and with the continuous growth of the national demand for animal blood products in recent years, some unscrupulous merchants are willing to shoddy and shoddy the real ones in order to make huge profits, such as duck Blood tofu is faked with cow blood and pig blood, which not only disrupts the market order, but also damages the interests and health of consumers. Research on the rapid identification technology of blood products can effectively protect the legitimate rights and interests of consumers and ensure the stable and healthy development of the market. [0003] Traditional blood product identification methods are...

Claims

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Application Information

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IPC IPC(8): G01N21/3577G01N21/359
CPCG01N21/3577G01N21/359
Inventor 杨勇林巍杨庆余李毛毛王存堂张舵董原
Owner QIQIHAR UNIVERSITY
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