Tissue culture method for artocarpus nanchuanensis

Abstract

The invention discloses a tissue culture method for artocarpus nanchuanensis. A tissue culture system for the artocarpus nanchuanensis is established; tissue culture explants of the artocarpus nanchuanensis are stem segments with buds, culture mediums for callus culture of the artocarpus nanchuanensis are WPM+2.0mg/L 2,4-D+0.2 or 0.3mg/L 6-BA+3g/L PVP, bud induction culture mediums are WPM+3.0mg/L6-BA+0.2mg/L NAA+3g/L PVP, and rooting culture mediums are WPM+1.5mg/L IBA+0.1mg/L NAA+3g/L PVP; the seedling exercising duration of tissue culture seedlings is 6-9 days, and sterilized nutritive soil is used. According to the tissue culture reproduction system established by the method disclosed by the invention, the callus rate of young leaves reaches 100%, the bud inductivity is 200%, the rooting rate reaches 75% or above, and the transplanting survival rate is 90% or above.

Description

technical field [0001] The invention belongs to the technical field of plant culture, and in particular relates to a method for tissue culture of Nanchuan wood pineapple. Background technique [0002] Nanchuan wood pineapple (Artocarpus nanchuanensis S.S.Chang) is a perennial tall tree plant, which is the plant of the genus Moraceae Jackfruit distributed in the northernmost part of my country. Also known as Nanchuan bread tree, water winter melon, melon scoop, and big bayberry. In 1989, Academician Wu Zhengyi and Professor Zhang Xiushi of the Kunming Institute of Botany, Chinese Academy of Sciences officially published a new species of Moraceae in the first issue of Volume 11 of Yunnan Botanical Research (Wu Zhengyi and Zhang Xiushi, 1989). In 2004, Nanchuan wood pineapple was identified as a "critically endangered" species by the "Red List of Species in my country", and it is one of the rarest endemic plants in China (Sun Rong, 2011). It is currently reported that it is m...

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Problems solved by technology

It can be propagated by cuttings, but the survival rate is low (Luo Hongguo et al., 2012)

Relevant units have conducted many years of investigation and research on this rare species resource. The seed propagation technology of Nanchuan wood pineapple has achieved some phased results (Sun Rong, 2011), and a cutting propagation method has been formed, but the survival rate of cuttings is only about 50%. (Luo Hongguo et al., 2013), its reproduction is affected by seasons, and requires a lot of land and labor, which is not conducive to large-scale production

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