Construction method of functional imitation cell outer layer membrane three-dimensional structure coating
A technology imitating the outer membrane of cells and a three-dimensional structure, applied in coatings and other directions, can solve problems such as unsatisfactory biocompatibility, and achieve the effects of convenient and flexible functionalization, wide application range, and simple use process
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[0016] 1. Preparation of polydopamine-mediated coating: prepare a 0.1-2 mg / mL dopamine aqueous solution (pH 7.5-8.6), and dip-coat it on the surface of the material to be modified at room temperature to form a stable polydopamine coating.
[0017] 2. On the surface of the polydopamine layer, construct a functional coating imitating the three-dimensional structure of the outer layer of the cell membrane:
[0018] 1) Immerse the polydopamine-coated material in an activated carboxypolyethylene glycol aqueous solution, and bond a designed amount of polyethylene glycol on the surface of polydopamine through amidation reaction to extend outward as a three-dimensional structure imitating the outer layer of the cell membrane flexible long chain;
[0019] 2) Then dip-coat in the aqueous solution of the imitation cell outer membrane structure copolymer (PMEN) containing the active ester group and the side chain of the phosphorylcholine group, and continue to coat the surface of the mate...
Embodiment 1
[0028]Example 1: Preparation of binary polymer PMEN containing active lipid groups and phosphorylcholine groups. Take a three-necked flask, add a dropping funnel, a reflux tube with a drying tube, and a device for venting nitrogen to the three ports respectively, add 20 ml of absolute ethanol to a 100 mL dry and clean three-necked flask, keep the temperature at 70°C, and keep the temperature for 30 min, weighed and added 0.05 g AIBN dissolved in 6 ml THF solution. 40 ml of anhydrous ethanol solution containing 1.05 g of active ester monomer MEONP and 2.98 g of phosphorylcholine group-containing monomer MPC were added dropwise, and the addition was completed within 4 h, and the reaction was carried out for 20 h. The resulting PMEN binary random copolymer solution was concentrated and put into a dialysis bag with a molecular weight cut-off of 7000, and dialyzed against pH 3.93 phosphoric acid aqueous solution for 48 h. PMEN flocculent solid was obtained after freeze-drying. 1 ...
Embodiment 2
[0029] Embodiment 2: the preparation that contains active ester functional polymer PMENP. Add 10 mL of absolute ethanol into a 100 mL three-necked flask, pass through nitrogen, stir with a magnetic force and gradually raise the temperature to 70 °C. Weigh 0.140 g of active ester monomer NPCEMA, 2.726 g of carboxyl chain monomer PEGA and 0.805 g of MPC in sequence, and add 34 mL of absolute ethanol to dissolve them. Weigh 0.037 gAIBN and add it to the monomer mixture. Use the dropping funnel to drop the mixed solution of the monomers, and the dropwise addition is completed in about 2 h. Change to a closed system and allow it to continue to react for 24 h. After the reaction was stopped, part of the reaction solution was taken out and put into a dialysis bag with a molecular weight cut off of 7000, and dialyzed in an acidic aqueous solution with a pH of 3~4. Freeze dried. D. 2 O solvent 1 H-NMR characterizes its structure, and its composition is polymer PMENP containing ac...
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