Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

CRK5 protein and application of coded gene thereof in regulating and controlling growth of plant stems and leaves

A technology for encoding genes and plant stems and leaves, which is applied in the application field of CRK5 protein and its encoding genes in regulating the growth of plant stems and leaves. Nutrients, the effect of broad application prospects

Inactive Publication Date: 2016-07-27
TSINGHUA UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under adverse environmental conditions such as short sunshine, weak light and too much rain, plants are prone to excessive growth, and this phenomenon gradually increases with the continuous deterioration of the natural environment, resulting in food production losses and economic losses.
The traditional method to deal with the excessive growth of stems and leaves mainly focuses on spraying growth regulators such as paclobutrazol and chlormequat to inhibit the excessive growth of stems and leaves, but this method has the disadvantages of high cost, heavy workload and environmental pollution.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • CRK5 protein and application of coded gene thereof in regulating and controlling growth of plant stems and leaves
  • CRK5 protein and application of coded gene thereof in regulating and controlling growth of plant stems and leaves
  • CRK5 protein and application of coded gene thereof in regulating and controlling growth of plant stems and leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, the acquisition and identification of CRK5 transgenic plants

[0052] The CRK5 gene involved in this example is derived from Arabidopsis thaliana (Arabidopsisthaliana), and its sequence in the Arabidopsis genome is shown in sequence 1 in the sequence table, and sequence 1 consists of 2537 nucleotides, which is the CRK5 The sequence of the gene in the Arabidopsis genome, wherein the 818-892, 1034-1103, 1226-1314, 1526-1599, 1838-1909 and 2064-2138 positions are all intron sequences; the cDNA sequence of the CRK5 gene is as follows As shown in sequence 2 in the sequence listing, sequence 2 is composed of 2082 nucleotides and is the cDNA sequence of the CRK5 gene, wherein the 1-1992 position is the coding sequence (ORF); both sequence 1 and sequence 2 are encoded in the sequence listing The protein shown in sequence 3, sequence 3 consists of 663 amino acid residues.

[0053] 1. Construction of recombinant expression vector pCAMBIA-1300-221-CRK5

[0054] The...

Embodiment 2

[0090] Embodiment 2, CRK5 transgenic plant seedling growth test analysis

[0091] ABA is an important signaling molecule for plants to resist external adversity stress, and has a wide range of physiological effects. ABA can promote the process of seed maturation and dormancy and the accumulation of storage proteins during seed maturation, inhibit seed germination and seedling growth, inhibit main root development, promote lateral root development, and promote leaf senescence and abscission. Therefore, in the presence of exogenous ABA, the root length and stem and leaf growth of plants are significantly inhibited. According to the sensitivity of wild-type and CRK5 transgenic plants to ABA, it can be detected whether CRK5 is involved in the process of ABA-inhibited stem and leaf growth.

[0092] The wild-type Arabidopsis (Col-0 ecotype), the single-copy inserted T obtained in Example 1 3 Seeds of homozygous OE-1 and OE-2 were sown directly on MS plates without ABA and containin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses CRK5 protein and application of a coded gene thereof in regulating and controlling the growth of plant stems and leaves.The application provided herein specifically includes: application of a protein (CRK4 protein) formed by amino acid sequences shown in sequence 3 of a sequence table, in the following items a1) or a2): a1), inhibiting excessive growth of the plant stems and leaves; a2), breeding a plant variety with the stem and leaf excessive growth inhibited.A CRK5 gene overexpressed plant is more sensitive to ABA-inhibited excessive growth of stems and leaves, as compared to wild control plants.The inhibition on the excessive growth of stems and leaves by regulating expression level of CRK5 is of great significance, and transgenic crops with high CRK5 gene expression and stems and leaves inhibited are acquired by means of genetic engineering.The purpose of sustainable development of agriculture is satisfied, it is possible to save nutrients and reduce the spraying of chemical fertilizers and pesticides, cultivation of green pollution-free new varieties is made possible, and the invention has a promising application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of a CRK5 protein and its coding gene in regulating the growth of plant stems and leaves. Background technique [0002] Crops or other plants, such as vegetables, flowers and fruit trees, often have overgrown stems and leaves, which can be called leggy stems and leaves. Under adverse environmental conditions such as short sunshine, weak light and excessive rain, plants are prone to excessive growth, and this phenomenon gradually increases with the continuous deterioration of the natural environment and the loss of food production and economic losses. The traditional method to deal with the excessive growth of stems and leaves mainly focuses on spraying growth regulators such as paclobutrazol and chlormequat to inhibit the excessive growth of stems and leaves, but this method has the disadvantages of high cost, heavy workload and environmental pollution. In recent years...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/12C12N15/54C12N15/82A01H5/00
CPCC12N9/1205C12N15/8261
Inventor 张大鹏路凯王小芳
Owner TSINGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products