CRK5 protein and application of coded gene thereof in regulating and controlling growth of plant stems and leaves
A technology for encoding genes and plant stems and leaves, which is applied in the application field of CRK5 protein and its encoding genes in regulating the growth of plant stems and leaves. Nutrients, the effect of broad application prospects
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Embodiment 1
[0051] Embodiment 1, the acquisition and identification of CRK5 transgenic plants
[0052] The CRK5 gene involved in this example is derived from Arabidopsis thaliana (Arabidopsisthaliana), and its sequence in the Arabidopsis genome is shown in sequence 1 in the sequence table, and sequence 1 consists of 2537 nucleotides, which is the CRK5 The sequence of the gene in the Arabidopsis genome, wherein the 818-892, 1034-1103, 1226-1314, 1526-1599, 1838-1909 and 2064-2138 positions are all intron sequences; the cDNA sequence of the CRK5 gene is as follows As shown in sequence 2 in the sequence listing, sequence 2 is composed of 2082 nucleotides and is the cDNA sequence of the CRK5 gene, wherein the 1-1992 position is the coding sequence (ORF); both sequence 1 and sequence 2 are encoded in the sequence listing The protein shown in sequence 3, sequence 3 consists of 663 amino acid residues.
[0053] 1. Construction of recombinant expression vector pCAMBIA-1300-221-CRK5
[0054] The...
Embodiment 2
[0090] Embodiment 2, CRK5 transgenic plant seedling growth test analysis
[0091] ABA is an important signaling molecule for plants to resist external adversity stress, and has a wide range of physiological effects. ABA can promote the process of seed maturation and dormancy and the accumulation of storage proteins during seed maturation, inhibit seed germination and seedling growth, inhibit main root development, promote lateral root development, and promote leaf senescence and abscission. Therefore, in the presence of exogenous ABA, the root length and stem and leaf growth of plants are significantly inhibited. According to the sensitivity of wild-type and CRK5 transgenic plants to ABA, it can be detected whether CRK5 is involved in the process of ABA-inhibited stem and leaf growth.
[0092] The wild-type Arabidopsis (Col-0 ecotype), the single-copy inserted T obtained in Example 1 3 Seeds of homozygous OE-1 and OE-2 were sown directly on MS plates without ABA and containin...
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