The invention discloses a special soilless culture nutrient solution for Brassica juncea and a preparation method of the special soilless culture nutrient solution. The special soilless culture nutrient solution for the Brassica juncea is prepared from macroelements, microelements and water, wherein the macroelements comprise nitrogen, phosphorus, potassium, calcium, magnesium and sulfur, and the microelements comprise iron, manganese, copper, zinc, boron and molybdenum. The special soilless culture nutrient solution for the Brassica juncea adopts reasonable composition, is nutrient-rich and low in cost, is prepared from the raw materials which are widely sourced and available and contains various nutrient elements and microelements needed for growth and development of the Brassica juncea, the Brassica juncea can grow healthily and strongly, the survival rate of Brassica juncea planting stocks is increased, fruit and vegetable quality is improved, so that the yield is increased, and the quality is improved; the preparation method is reasonable, adopts simple and easy process, is high in efficiency, low in application cost, simple to operate and easy to implement, has good application prospect and is convenient to use, and the application is convenient and fast.