Method of using coconut cake globulin as raw material to prepare ACE inhibiting peptide

A technology of globulin and inhibitory peptides, which is applied in the field of preparation of coconut bran globulin, ACE inhibitory peptides, and preparation of ACE inhibitory peptides, can solve the problems of waste of resources, active ingredients such as proteins have not been developed and utilized, and achieve the effect of promoting development

Inactive Publication Date: 2016-07-27
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, coconut bran is only used as an auxiliary material for food such as animal feed or cakes, and ...

Method used

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  • Method of using coconut cake globulin as raw material to prepare ACE inhibiting peptide
  • Method of using coconut cake globulin as raw material to prepare ACE inhibiting peptide
  • Method of using coconut cake globulin as raw material to prepare ACE inhibiting peptide

Examples

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Effect test

Embodiment 1

[0024] 1. Pre-treatment of raw materials Select mature old coconut fruits, then remove the shells, peel off the seed coat, wash, grate, and squeeze the juice to obtain coconut bran. Coconut bran is dried at 45°C, crushed, passed through a 60-mesh sieve, and then petroleum ether or ether is added at a ratio of 1:10 (m / v) to extract the remaining coconut oil to obtain defatted coconut bran powder.

[0025] 2. To extract globulin, add 0.4mol / L sodium chloride solution to the defatted coconut bran powder at a ratio of 1:10 (m / v), shake and extract at room temperature for 2 hours, filter, and collect the filtrate. Add the above-mentioned sodium chloride solution (1:10, m / v) to the filter residue again, repeat the extraction 3 times according to the above method, and combine the collected filtrates. The filtrate was centrifuged at 4° C. and 12000 rpm for 15 min, and the supernatant was collected. Put this supernatant into a dialysis membrane with a molecular weight cut off ...

Embodiment 2

[0031] 1. Pre-treatment of raw materials Select mature old coconut fruits, then remove the shells, peel off the seed coat, wash, grate, and squeeze the juice to obtain coconut bran. Coconut bran is dried at 50°C, crushed, passed through a 60-mesh sieve, and then petroleum ether or ether is added at a ratio of 1:15 (m / v) to extract the remaining coconut oil to obtain defatted coconut bran powder.

[0032] 2. To extract globulin, add 0.4mol / L sodium chloride solution to the defatted coconut bran powder at a ratio of 1:10 (m / v), shake and extract at room temperature for 3 hours, filter, and collect the filtrate. Add the above-mentioned sodium chloride solution (1:15, m / v) to the filter residue again, repeat the extraction 3 times according to the above method, and combine the collected filtrates. The filtrate was centrifuged at 4° C. and 10000 rpm for 20 min, and the supernatant was collected. Put the supernatant into a dialysis membrane with a molecular weight cut-off o...

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Abstract

The invention relates to a method of using coconut cake globulin as a raw material to prepare ACE inhibiting peptide. The method includes: selecting mature old coconut, and treating the same to obtain degreased coconut cake powder; extracting coconut cake globulin, and performing compound enzyme hydrolysis and centrifugal separation to prepare polypeptide original extract; separating out five polypeptide components from the polypeptide original extract, collecting the fifth polypeptide component, and further separating and purifying to obtain 12 polypeptide components; further subjecting the ninth component to carbon 18 analytical column-reversed phase high performance liquid chromatographic separation to obtain two pure peptides, wherein ACE inhibition rate of each pure peptide is higher than 70%. The coconut cake globulin is used as a substrate, the ACE inhibiting peptide higher than 95% in purity is prepared by adopting technologies of compound enzymolysis, gel chromatography separation and reversed phase high performance liquid chromatography purification, the ACE inhibiting peptide can be used as an ingredient of natural anti-hypertension drugs, can be added into food to prevent and treat food-borne hypertension, is conducive to realizing comprehensive utilization and deep processing of coconut and can powerfully promote development of coconut industry of China.

Description

technical field [0001] The invention belongs to the technical field of post-harvest processing of agricultural products, and relates to a method for ACE inhibitory peptides, in particular to a method for preparing coconut bran globulin from coconut bran as a raw material, and further preparing ACE inhibitory peptides. Background technique [0002] Hypertension is one of the major diseases threatening human health. According to statistics, the average incidence rate of hypertension in various countries in the world is 10-20%. The number of deaths due to hypertension in the world reaches 12 million each year, and the number of people who die of hypertension and related diseases in China reaches 2 million each year. Hypertension has become an urgent problem One of the world's health problems. [0003] Angiotensin converting enzyme (AntiotensinIConvertingEnzyme, ACE) plays a key role in regulating blood pressure. Studies have shown that the renin-angiotensin system (RAS) and k...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/415C07K1/20C07K1/16
CPCC07K14/415C12P21/06
Inventor 郑亚军李艳张玉峰陈华赵松林
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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